Table 7.

Association of FISH and immunohistochemistry data and outcome

Best radiographic response
PR/SD (%)*PD (%)Total (%)*
FISH
    EGFR amplified3 (50)3 (50)6 (43)
    EGFR not amplified3 (38)5 (63)8 (57)
    PTEN loss4 (57)3 (43)7 (50)
    PTEN no loss2 (29)5 (71)7 (50)
Immunohistochemistry§
    EGFR high3 (33)6 (67)9 (90)
    EGFR low1 (100)01 (10)
    p-S6 high2 (33)4 (67)6 (60)
    p-S6 low2 (50)2 (50)4 (40)
    p-MAPK high3 (50)3 (50)6 (60)
    p-MAPK low1 (25)3 (75)4 (40)
    p-AKT high4 (44)5 (56)9 (90)
    p-AKT low
01 (10)1 (10)
  • Abbreviations: PR, partial response; SD, stable disease; PD, progressive disease.

  • * % Patients with an evaluable assay.

  • Amplified: EGFR/chromosome 7 centromere ratio of >2.0.

  • Loss: 30% of nuclei with definitive loss and 20-30% with indeterminate loss.

  • § High, 1-4+ intensity in ≥25% of tumor cells; low, either no staining or 1-4+ intensity in <25% of tumor cells.