Table 2.

Biological characteristics of 21 HBC xenografts originating from 18 patients

XenograftHistologyp53RB*P-AKT*PTEN*VEGF*ER*PRHER1 (mRNA)p-HER1* (protein)HER2 (mRNA)p-HER2* (protein)HER3HER4Ki67
HBCx-1ICCMut±±++±780560+++4,416++310757,724
HBCx-2ICCMut±+++++++±010++970±462676,514
HBCx-3ICCMut+++++++++++±1,38116630563±5187085,592
HBCx-18ICCMut±++++++++000ND0ND0NDNDND
HBCx-4AICCMut++++++++++ND32505,221+++460+++170128,046
HBCx-4B
HBCx-5§ICCWT++++±+98601++++78,607++++2,7381,19315,326
HBCx-6ICCMut+++++NDND3401,500++1,480++1921064,507
HBCx-7ILCWT+0++0004,726+++370±124010,861
HBCx-8ICCWT+++++±0106480676+5361811,528
HBCx-9ICCMut±0+++201,365++1,762+1,0747511,484
HBCx-10ICCMut±0±01900673++1,622+1,119216,405
HBCx-11ICCMut++0±+002,195+++1,347++3571443,509
HBCx-13AICCMut+++++++++444320++++30,595+++9561,4681,140
HBCx-13B++++++
HBCx-14ICCMut000+++350283+++301+344194,158
HBCx-15ICCMut++0+++1501,080++1,056++205,484
HBCx-12A§ICCMut++++++±0671954±5,548+1,960457,040
HBCx-12B§0±+
HBCx-19ILCMut++++++++010ND+++ND++NDNDND
HBCx-16ICCMut+++±++++1103,138+++7,119+9761247,055
  • NOTE: p53 mutations were determined by Fasay Assay. High expression levels are indicated in bold characters.

  • * Rb, PTEN, P-AKT, p-HER1, HER2 expression (semiquantitative) are by Western blotting analysis; the other genes are by quantitative RT-PCR.

  • Overexpression limit, 1,000 units.

  • From metastases, all others from primary tumors.

  • § Treated before graft.