Table 3.

Competitive binding assay of p53 mutant peptides to DQ7 (DQA1*0301, DQB1*0301, DQB 3.1)

PeptideIC50 (umol/L)
AYK (positive control)<0.1
34P (negative control)>10
p53 mutant patient 1680.58
p53 wild-type patient 1684.5
p53 mutant patient 69>10
p53 wild-type patient 69>10
p53 mutant patient 226>10
p53 wild-type patient 226>10
  • NOTE: p53 peptides, both mutant and wild type, were sequenced (14-mers), and IC50 was determined.