Table 2.

CD4+ T cells and CD8+ T-cell responses upon stimulation with peptide pools of HPV16

A. Percentages of IFNγ producing CD4+ and CD8+ T cells
PatientCD4
CD8
CD4
CD8
E6.1
E6.2
E6.3
E6.4
E6 prot
E6
E7.1
E7.2
E7 prot
E7
PrePostPrePostPrePostPrePostPrePostPrePostPrePostPrePostPrePostPrePost
ID1000.090.060.110.010.010.09 (aa 51-69)0.03
ID1020.090.210.220.320.120.770.431.32 (aa 51-69)0.020.090.330.030.35 (aa 51-69)
ID1030.100.390.550.461.120.040.600.70 (aa 11-29)
ID1050.050.050.020.460.080.92 (aa 51-69)
0.83 (aa 61-79)
ID1060.450.271.000.640.114.552.730.30 (aa 41-59)0.021.010.230.05 (aa 1-19)
0.83 (aa 51-69)0.10 (aa 41-59)
1.37 (aa 131-149)
ID1070.161.590.220.940.280.08 (aa 91-109)0.120.21 (aa 71-89)
B. Percentages of CD4+CD25+ Foxp3-negative and Foxp3-positive cells
Patient
CD4+CD25+
Foxp3
Foxp3+
PrePostPrePost
ID100E60.321.960.41.29
E70.680.480.48
ID102E614.53.3
E70.691.810.11.37
ID103E60.290.722.01
E70.380.281
ID105E60.2235.41.943.1
E71.61
ID106E60.3939.41.2210.2
E71.992.47
ID107E61.1813.50.872.91
E70.280.621.93
  • NOTE: (A) The percentage of IFNγ-producing T cells upon stimulation with pools of 22-mer peptides (CD4) or pools of 10-mer peptides (CD8) is indicated. For the CD8+ T-cell responses, the number of the first and last amino acid of the HPV16 antigen that is covered by the peptide pool recognized is given. The results of both before vaccination and only the positive responses after the last vaccination are shown.

    (B) The percentages of HPV16 peptide stimulated CD4+CD25+ T cells which do not (Foxp3) or do express Foxp3 (Foxp3+) after subtraction of the percentage of cells in nonstimulated (medium only) cultures are given.

    Abbreviations: Pre, before vaccination; Post, after the last vaccination; aa, amino acid.