Table 2.

A list of identified protein

Spots no*Accession noIdentified proteinWilcoxon test P valueFold difference (ration of means)Overall RankpI (obs)pI (cal)§MW (obs; kDa)MW (cal; kD)§Protein scorePeptide matchesSequence coverage (%)
1378Q96CX2Potassium channel tetramerisation domain containing protein 12 (Pfetin)8.23E-054.86216.45.535.135.7406820.0
1314Q96CX2Potassium channel tetramerisation domain containing protein 12 (Pfetin)8.23E-055.80525.45.544.035.7338719.7
1,371Q96CX2Potassium channel tetramerisation domain containing protein 12 (Pfetin)8.23E-053.78156.25.535.735.714628.3
1413Q96CX2Potassium channel tetramerisation domain containing protein 12 (Pfetin)1.65E-043.198266.55.534.535.7306823.4
575P17987T-complex protein 1, α subunit3.29E-041.405285.15.873.360.3267612.4
893O00148ATP-dependent helicase DDX396.99E-042.321415.55.551.849.110923.7
1367P11177Pyruvate dehydrogenase estrone component β subunit8.94E-043.669246.46.235.139.217439.7
1303Q96CX2Pfetin9.87E-042.91745.25.544.735.7236514.5
1919P00441Superoxide dismutase (Cu-Zn)1.33E-032.547126.65.715.315.86017.8
611P17987T-complex protein 1, α subunit1.56E-031.98294.95.867.960.36671122.5
1184P60709Actin, cytoplasmic 11.56E-031.902205.95.346.341.7276411.2
1376Q96CX2Pfetin1.56E-032.850386.55.535.135.73961123.7
834P14625Endoplasmin precursor1.75E-033.48784.74.857.292.59771820.5
483P26038Moesin1.86E-031.558155.26.184.067.720846.9
369P08238Heat shock protein HSP 90-β1.86E-031.977164.45.089.483.126357.3
922P05091Aldehyde dehydrogenase, mitochondrial precursor2.04E-031.359315.66.650.056.4323510.1
273Q9Y4L1150 kDa oxygen-regulated protein precursor3.32E-031.384364.75.2113.7111.310222.4
1436P07355Annexin A23.32E-031.590396.97.634.538.5262513.3
1,748P62993Growth factor receptor-bound protein 23.32E-031.545406.05.925.525.27927.4
205P12814α-actinin 13.70E-031.993114.45.3114.1103.15931212.9
1710P04792Heat-shock protein β-13.70E-032.078136.16.027.522.84911554.1
574P20700Lamin B13.70E-032.575144.25.175.166.316163336.4
1555P08758Annexin A53.70E-031.438196.14.931.535.89625.3
454P26038Moesin3.70E-031.441375.46.182.367.736689.2
1315P14625Endoplasmin precursor3.73E-032.895235.54.844.392.521654.7
1276Q9Y3F4Serine-threonine kinase receptor-associated protein3.85E-031.650295.15.045.038.4250515.4
304Q01432AMP deaminase 34.00E-031.691224.86.5113.488.818534.6
1372Q96CX2Pfetin4.04E-032.972306.35.535.135.711828.3
1478P40925Malate dehydrogenase, cytoplasmic4.08E-031.534276.26.933.636.311625.7
1916P09382Galectin-14.21E-031.91776.15.316.914.6244635.8
1209P30740Leukocyte elastase inhibitor4.33E-032.380185.25.946.042.7551923.0
1884P00441Superoxide dismutase (Cu-Zn)5.25E-033.12136.85.718.415.8139217.0
77P42704130 kDa leucine-rich protein5.51E-031.456214.55.5195.2145.212021.7
1166P60709Actin, cytoplasmic 15.51E-033.971425.95.346.341.7246715.5
1921P09382Galectin-16.22E-031.91266.15.315.814.690311.9
1421Q96CX2Pfetin6.22E-031.665346.45.534.535.73361323.4
366P08238Heat shock protein HSP 90-β7.02E-031.705254.45.089.483.1420813.8
1566P82979Nuclear protein Hcc-17.02E-031.526436.16.130.623.5196213.9
473P26038Moesin7.59E-031.323325.66.180.567.77751518.8
1205Q9NYL9Tropomodulin-37.90E-031.935105.25.146.039.616839.1
283P08238Heat shock protein HSP 90-β7.90E-031.300334.15.0113.183.19071722.1
463P26038Moesin7.90E-031.524355.46.182.367.735679.7
841P14625Endoplasmin precursor9.45E-032.478174.74.857.292.515934.0
  • * Spot numbers refer to those in Fig. 1A and Supplementary Fig. S3.

  • Accession numbers of protein were derived from SWISS-PROT and National Center for Biotechnology Information nonredundant databases.

  • Observed isoelectric point and molecular weight calculated according to location on the two-dimensional gel.

  • § Theoretical isoelectric point and molecular weight obtained from Swiss-Prot and the ExPASy database. (http://au.expasy.org).

  • Mascot score for the identified proteins based on the peptide ions score (P < 0.05) (http://www.matrixscience.com).