Table 1.

IC50 of daunorubicin and geldanamycin in pancreatic cancer cells under normoxia and hypoxia

Human pancreatic cancer cell linesDaunorubicinGeldanamycin
Normoxia IC50 (umol/L)Hypoxia IC50 (umol/L)RINormoxia IC50 (umol/L)Hypoxia IC50 (umol/L)RI
BxPC-30.33 ± 0.110.95 ± 0.132.89 ± 1.030.85 ± 0.120.13 ± 0.110.15 ± 0.13
Panc-12.52 ± 0.327.74 ± 0.43.1 ± 0.42>104.4 ± 2.2<0.4
MiaPaCa-20.016 ± 0.0170.026 ± 0.0161.63 ± 2.06.5 ± 3.50.71 ± 0.380.12 ± 0.08
  • NOTE: The cytotoxicity of daunorubicin and geldanamycin under both normoxia and hypoxia were measured by MTS assay in three independent experiments with six replicates in each experiment. Relative cell viability was calculated by normalized intensity to nontreatment cell control under different drug concentrations after 72 h of incubation. IC50s were calculated by WinNonlin 5.0. RI induced by hypoxia was calculated as the ratio of IC50 under hypoxia and IC50 under normoxia.