Table 1

Sequences of primers used for multiplex RT-PCR detection of cytokines

Flt3(stk-1)5′ primer: 5′-TCA-AGT-GCT-GTG-CAT-ACA-ATT-CCC-3′
3′ primer: 5′-CAC-CTG-TAC-CAT-CTG-TAG-CTG-GCT-3′208 bp
SCF5′ primer: 5′-CTG-CTC-CTA-TTT-AAT-CCT-CTC-GTC-3′
3′ primer: 5′-CAC-AAG-GTC-ATC-CAC-TAT-ATT-CAC-3′291 bp
IL-1RA5′ primer: 5′-ATG-GAA-ATC-TGC-AGA-GGC-CTC-3′
3′ primer: 5′-GTC-CTG-CTT-TCT-GTT-CTC-GCT-3′360 bp
GM-CSF5′ primer: 5′-ATG-TGG-CTG-CAG-AGC-CTG-CTG-C-3′
3′ primer: 5′-CTG-GCT-CCC-AGC-AGT-CAA-AGG-G-3′424 bp
IL-65′ primer: 5′-ATG-AA-C-TCC-TTC-TCC-ACA-AGC-GC-3′
3′ primer: 5′-GAA-GAG-CCC-TCA-GGC-TGG-ACT-G-3′628 bp
TNF-α5′ primer: 5′-ATG-AGC-ACT-GAA-AGC-ATG-ATC-CGG-3′
3′ primer: 5′-GCA-ATG-ATC-CCA-AAG-TAG-ACC-TGC-3′695 bp
IL-1β5′ primer: 5′-ATG-GCA-GAA-GTA-CCT-AAG-CTC-GC-3′
3′ primer: 5′-CAC-AAA-TTG-CAT-GGT-GAA-GTC-AG-3′802 bp
β-Actin mRNA5′ primer: 5′-GGG-TCA-GAA-GGA-TTC-CTA-TG-3′
3′ primer: 5′-CTA-GAA-GCA-TTT-GCG-GTG-GAC-3′1000 bp