Table 2

Reproducibility of the CK20 relative ratio

All values expressed as mean (coefficient of variation).
Amount of RNA
Calibrator (10 ng; n = 9)aSample
1 ng (n = 9)10 ng (n = 9)100 ng (n = 9)All (n = 27)
CK20 crossing point24.69 (0.5%)28.09 (1.2%)25.05 (1.0%)22.80 (0.7%)
PBGD crossing point26.72 (0.4%)30.55 (0.8%)27.52 (0.9%)25.18 (0.7%)
CK20 copy number15,441 (17%)1452 (30%)11,982 (18%)58,159 (15%)
PBGD copy number1763 (13%)213 (26%)1134 (13%)4131 (9%)
CK20 copies/ng RNA (Standard curve)154 (17%)1452 (30%)1198 (18%)581 (15%)1077 (43%)
CK20 relative ratio (Standard curve)1,000,000787,135 (21%)1,211,483 (12%)1,616,892 (13%)12,050,170 (24%)
CK20 relative ratio (Efficiency = 2)1,000,0001,360,699 (16%)1,351,985 (9%)1,286,643 (13%)1,333,109 (12%)
CK20 relative ratio (Efficiency adjusted)1,000,0001,361,204 (17%)1,378,494 (10%)1,327,308 (14%)1,355,669 (13%)
  • a n, number of determinations.