Table 1

Sequences of primers and hybridization probes used in this study

GeneUseNameOligonucleotide sequence (5′-3′)
CK-19Forward primerCK19-forgCACTACAgCCACTACTACACgA
Reverse primerCK19-doCTCATgCgCAgAgCCTgTT
Hybridization probeCK19-FLaTgTCCTgCAgATCgACAACgCCC-FL
Hybridization probeCK19-LCbLCRed640-CTggCTgCAgATgACTTCCgAACC
GAPDHForward primerGAPDH FTTggTATCgTggAAggACTCA
Reverse primerGAPDH RTgTCATCATATTTggCAggTTT
Hybridization probeGAPDH 3FLaTgTCCCCACTgCCAACgTgTCAg-FL
Hybridization probeGAPDH 705cLCRed705-ggTggACCTgACCTgCCgTCTAgA
  • a Labeled with fluorescein.

  • b Labeled with LC Red640 (TIB MOLBIOL).

  • c Labeled with LC Red705 (TIB MOLBIOL).