Article Figures & Data
Figures
- Fig. 1.
Tranfection of fas into the SAOS-LM7 cell line. A. LM7 cells and LM7 cells stably transfected with fas (LM7-Fas; 2.5 × 105) or a neomycin control plasmid (LM7-neo) were stained with biotinylated antibodies to Fas, treated with streptavidin-phycoerythrin, and analyzed by flow cytometry. B. The above-mentioned cell lines were plated on a 96-well plate in 250 μL of medium and treated as indicated with medium alone (▪), 1 ng/mL Fas antibody (CH11;
), or 1 ng/mL IgM isotype control (). After 60 hours, growth inhibition was assayed by [3H]thymidine incorporation. - Fig. 2.
Effect of fas transfection on the metastatic potential of LM7 cells. Mice received intravenous injection with 1 × 106 LM7 cells, LM7 neo transfectants (LM7-neo), or LM7 fas transfectants (LM7-Fas). The mice were sacrificed 10 weeks later, the lungs were fixed in Bouin’s solution, and the metastases were counted. LM7-Fas cells resulted in fewer nodules compared with LM7 cells (P = 0.0002) and LM7-neo cells (P < 0.0001).
- Fig. 3.
Fas expression in lung metastases from patients with osteosarcoma. Paraffin-embedded tissue sections were prepared and stained as described in Materials and Methods. Representative slides from patients 1, 8, 13, and 16 are shown. Anti-Fas immunoreactivity is shown as the brown-stained areas, whereas areas that were not reactive to the anti-Fas antibody are indicated by the blue counterstain (hematoxylin). Arrows indicate positive staining for Fas in the patient sample. Human liver was used as a positive control. Original magnification, ×100.
Tables
- Table 1
Effect of Fas transfection on the metastatic potential of LM7 cells
LM7 7.0/>200 5.5/>200 6.0/>200 5.5/>200 6.5/>200 9.0/>200 LM7-neo >0.5/170 >0.5/128 >0.5/49 4.0/121 2.5/167 >0.5/15 >0.5/37 >0.5/8 >0.5/20 >0.5/28 LM7-Fas 0/0 <0.5/1 <0.5/2 <0.5/3 0/0 <0.5/2 0/0 <0.5/3 0/0 <0.5/4 -
NOTE. Mice received intravenous injection with 1 × 106 LM7 cells, LM7 neo transfectants (LM7-neo), or LM7 fas transfectants (LM7-Fas). Ten weeks later, the mice were sacrificed, the lungs were fixed in Bouin’s solution, and the metastases were counted and measured. Data represent size (in mm)/number of nodules.
-
Volume 10, Issue 23
