Article Figures & Data
Figures
- Fig. 1.
A, unsupervised analysis using multidimensional scaling based on the overall gene expression in endometrioid (green) and papillary serous (blue) using 1-correlation as distance metric of 18.4 K transcripts detected in at least 50% of the arrays. B, differentially expressed genes between 66 endometrioid carcinomas and 24 papillary serous carcinomas. Twenty-five most up-regulated and 25 most down-regulated genes at P < 0.001. Each sample in the heat map is labeled histology, stage of disease, and coded tumor number. The heat map was color coded using red for up-regulation from normal endometria and green for down-regulation.
- Fig. 2.
A, unsupervised analysis using multidimensional scaling based on the overall gene expression in endometrioid carcinomas (red) and mixed mullerian tumor (blue). B, genes differentially expressed between 66 endometrioid carcinomas (E) and 29 mixed mullerian tumors (MMT) of the uterus. Twenty-five most up-regulated and 25 most down-regulated genes at P < 0.001. Each sample in the heat map is labeled histology, stage of disease, and coded tumor number. The heat map was color-coded using red for up-regulation from normal endometria and green for down-regulation.
- Fig. 3.
A, unsupervised analysis using multidimensional scaling based on the overall gene expression in mixed mullerian tumor (red) and papillary serous carcinoma (blue). B, list of 25 highest and 25 lowest differentially expressed genes (at least 2-fold) for 29 mixed mullerian tumors and 24 papillary serous (PS) carcinomas (P < 0.001). Each sample in the heat map is labeled histology, stage of disease, and coded tumor number. The heat map was color coded using red for up-regulation from normal endometria and green for down-regulation.
- Fig. 4.
Microarray expression and quantitative PCR (TaqMan) expression analysis of six selected genes differentially expressed between mixed mullerian tumors, endometrial carcinoma, and papillary serous carcinoma.
Tables
- Table 1. Differentially expressed genes characteristic of endometrioid serous carcinomas
Differentially expressed genes characteristic of endometrioid serous carcinomas
E/PS E/MMT UniGene Gene Map Description 8.027 19.501 Hs.104696 KIAA1324 1p13.3-p13.2 Maba1 6.279 5.010 Hs.420036 GAD1 2q31 Glutamate decarboxylase 1 (brain, 67 kDa) 5.819 19.348 Hs.115838 16p13.11 Homo sapiens cDNA FLJ44282 fis, clone TRACH2003516 5.745 9.057 Hs.82961 TFF3 21q22.3 Trefoil factor 3 (intestinal) 5.603 16.175 Hs.226391 AGR2 7p21.3 Anterior gradient 2 homologue (Xenopus laevis) 4.989 8.530 Hs.155651 FOXA2 20p11 Forkhead box A2 4.627 8.343 Hs.48403 FLJ10847 17p11.2 Hypothetical protein FLJ10847 4.204 4.452 Hs.46452 SCGB2A2 11q13 Secretoglobin, family 2A, member 2 3.807 8.202 Hs.24879 PPAP2C 19p13 Phosphatidic acid phosphatase type 2C 3.784 8.290 Hs.145807 TMC5 16p13.11 Transmembrane channel–like 5 3.692 5.524 Hs.104696 KIAA1324 1p13.3-p13.2 Maba1 3.617 3.865 Hs.99348 DLX5 7q22 Distal-less homeo box 5 3.406 7.984 Hs.200286 DKFZp547I048 1p31.1 Hypothetical protein DKFZp547I048 3.223 6.239 Hs.6168 KIAA0703 16q24.1 KIAA0703 gene product 3.185 4.521 Hs.158857 RASSF6 4q21.21 Ras association (RalGDS/AF-6) domain family 6 3.172 4.746 Hs.445072 ARGBP2 4q35.1 Arg/Abl-interacting protein ArgBP2 3.107 3.655 Hs.89404 MSX2 5q34-q35 Msh homeo box homologue 2 (Drosophila) 2.633 4.250 Hs.512682 CEACAM1 19q13.2 Carcinoembryonic antigen–related cell adhesion molecule 1 2.589 3.198 Hs.288240 IL20RA 6q22.33-q23.1 Interleukin 20 receptor α 2.572 8.822 Hs.116992 HGD 3q21-q23 Homogentisate 1,2-dioxygenase (homogentisate oxidase) 2.547 2.755 Hs.211587 PLA2G4A 1q25 Phospholipase A2, group IVA (cytosolic, calcium-dependent) 2.487 2.187 Hs.306278 CD44 11p13 CD44 antigen (homing function and Indian blood group system) 2.470 2.941 Hs.156880 4 Homo sapiens, clone IMAGE:4791597, mRNA 2.458 3.778 Hs.519137 4 Homo sapiens cDNA FLJ37284 fis, clone BRAMY2013590 2.456 4.519 Hs.432615 Homo sapiens transcribed sequence 2.428 2.310 Hs.11713 ELF5 11p13-p12 E74-like factor 5 (Ets domain transcription factor) 2.420 3.915 Hs.145807 TMC5 16p13.11 Transmembrane channel–like 5 2.383 2.065 Hs.348802 NMA 10p12.3-p11.2 Putative transmembrane protein 2.265 2.581 Hs.306278 CD44 11p13 CD44 antigen (homing function and Indian blood group system) 2.218 8.166 Hs.433197 ASRGL1 11q12.3 Asparaginase like 1 2.215 2.271 Hs.153952 NT5E 6q14-q21 5′-Nucleotidase, ecto (CD73) 2.184 2.901 Hs.79414 PDEF 6p21.3 Prostate epithelium–specific Ets transcription factor 2.124 3.088 Hs.439760 CYP4X1 1p33 Likely orthologue of rat cytochrome P450 4X1 2.121 2.453 Hs.293685 Homo sapiens transcribed sequence 2.096 3.305 Hs.518542 HGD 3q21-q23 Homogentisate 1,2-dioxygenase (homogentisate oxidase) 2.073 4.648 Hs.194710 GCNT3 15q21.3 Glucosaminyl (N-acetyl) transferase 3, mucin type 0.485 0.411 Hs.183650 CRABP2 1q21.3 Cellular retinoic acid binding protein 2 0.479 0.498 Hs.12844 EGFL6 Xp22 EGF-like domain, multiple 6 0.472 0.485 Hs.308628 SIAT8D 5q21 Sialyltransferase 8D (-2, 8-polysialyltransferase) 0.465 0.384 Hs.298646 PRO2000 8q24.13 PRO2000 protein 0.454 0.451 Hs.414407 HEC 18p11.31 Highly expressed in cancer, rich in leucine heptad repeats 0.450 0.279 Hs.336224 TMEFF1 9q31 Transmembrane protein with EGF-like 0.444 0.469 Hs.137047 FLJ25157 3p24.2 Hypothetical protein FLJ25157 0.443 0.193 Hs.76118 UCHL1 4p14 Ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) 0.441 0.426 Hs.445098 FLJ20354 1p31.2 Hypothetical protein FLJ20354 0.437 0.381 Hs.169840 TTK 6q13-q21 TTK protein kinase 0.430 0.364 Hs.125124 EPHB2 1p36.1-p35 EphB2 0.423 0.349 Hs.79440 IMP-3 7p11 IGF-II mRNA-binding protein 3 0.407 0.315 Hs.250687 TRPC1 3q22-q24 Transient receptor potential cation channel, subfamily C 0.384 0.421 Hs.444096 Human full-length cDNA 5′-end of clone CS0DK007YB08 0.382 0.382 Hs.65366 KIAA1495 Xq24 KIAA1495 protein 0.370 0.435 Hs.132246 SLC38A1 12q13.11 Solute carrier family 38, member 1 0.358 0.311 Hs.444096 Human full-length cDNA 5′-end of clone CS0DK007YB08 0.346 0.264 Hs.512414 NR3C1 5q31 Nuclear receptor subfamily 3C1 (glucocorticoid receptor) 0.337 0.322 Hs.30299 IMP-2 3q28 IGF-II mRNA-binding protein 2 0.308 0.199 Hs.43577 ATP8B2 1q22 ATPase, class I, type 8B, member 2 0.306 0.298 Hs.444096 Human full-length cDNA 5′-end of clone CS0DK007YB08 0.302 0.135 Hs.126085 LRRN1 3p26.2 Leucine-rich repeat neuronal 1 0.276 0.210 Hs.14968 PLAG1 8q12 Pleiomorphic adenoma gene 1 0.259 0.104 Hs.349109 IGF2 11p15.5 Insulin-like growth factor 2 (somatomedin A) 2.042 2.679 Hs.387367 CYP39A1 6p21.1-p11.2 Cytochrome P450, family 39, subfamily A, polypeptide 1 2.035 3.538 Hs.184510 SFN 1p35.3 Stratifin 2.031 2.162 Hs.302738 SLC26A2 5q31-q34 Solute carrier family 26 (sulfate transporter), member 2 2.031 2.372 Hs.105547 NPDC1 9q34.3 Neural proliferation, differentiation and control, 1 2.030 3.485 Hs.28491 SAT Xp22.1 Spermidine/spermine N1-acetyltransferase 2.017 2.669 Hs.302738 SLC26A2 5q31-q34 Solute carrier family 26 (sulfate transporter), member 2 Abbreviations: E, endometrioid carcinoma; PS, papillary serous carcinoma; MMT, mixed mullerian tumors.
- Table 2. Differentially expressed genes characteristic of papillary serous carcinoma
Differentially expressed genes characteristics of papillary serous carcinoma
PS/E PS/MMT Gene UniGene Map Description 7.127 9.804 VGLL1 Hs.9030 Xq26.3 Vestigial like 1 (Drosophila) 4.860 8.945 Hs.147613 Homo sapiens transcribed sequences 4.292 7.194 Hs.372225 Homo sapiens transcribed sequences 3.757 3.366 NPR1 Hs.438864 1q21-q22 Natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A) 3.254 3.357 LOC221002 Hs.125293 10q11.21 CG4853 gene product 3.187 2.580 FAM20A Hs.144633 17q24.3 Family with sequence similarity 20, member A 3.113 2.533 ESTs, weakly similar to JC5314 CDC28/cdc2-like kinase associating arginine-serine cyclophilin 2.854 3.476 WNT7A Hs.72290 3p25 Wingless-type mouse mammary tumor virus integration site family, member 7A 2.789 3.011 THRB Hs.203213 3p24.3 Thyroid hormone receptor β [erythroblastic leukemia viral (v-erb-a) oncogene homologue 2, avian] 2.545 2.704 Hs.391828 Homo sapiens transcribed sequence with moderate similarity to protein NP_060265.1 2.379 3.736 PTGS1 Hs.88474 9q32-q33.3 Prostaglandin endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) 2.363 2.013 LU Hs.155048 19q13.2 Lutheran blood group (Auberger B antigen included) 2.315 2.977 PTGS1 Hs.88474 9q32-q33.3 Prostaglandin endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) 2.277 2.748 PTGS1 Hs.88474 9q32-q33.3 Prostaglandin endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) 2.255 2.010 CDH6 Hs.32963 5p15.1-p14 Cadherin 6, type 2, K-cadherin (fetal kidney) 2.240 3.697 Hs.201441 13 Homo sapiens cDNA FLJ11076 fis, clone PLACE1005077. 2.187 3.340 HUMPPA Hs.78358 17q25.2 Paraneoplastic antigen 2.178 2.477 KIAA1554 Hs.195642 17q25.3 KIAA1554 protein 2.097 2.479 Hs.152422 Homo sapiens cDNA FLJ27210 fis, clone SYN03494 2.023 2.317 Hs.124776 Homo sapiens mRNA; cDNA DKFZp564N1116 (from clone DKFZp564N1116) 0.480 0.396 FGFR1 Hs.748 8p11.2-p11.1 Fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome) 0.449 0.468 KIAA1025 Hs.435249 12q24.22 KIAA1025 protein
Volume 11, Issue 11
