Fig. 2.
Overview of interphase FISH results on formalin-fixed paraffin-embedded tissue. A, a case showing EWSR1 rearrangement by interphase FISH using EWSR1 break-apart probe (Zymed). Proximal and distal EWSR1 regions are hybridized by separate probes (green and red, respectively). The presence of separate green and red signals indicates a rearrangement of the EWSR1 region on chromosome 22 (arrows), whereas colocalized signals represent the normal EWSR1 region on chromosome 22 (arrowhead). B, the same case as in (A) with CREB1 rearrangement. The distal CREB1 region is hybridized to two contiguous fosmid probes, G248P81788B12 and G248P89268A6, labeled with biotin-11-dUTP and revealed by Cy3-labeled streptavidin (red), whereas the proximal CREB1 region is hybridized to the BAC probe RP11-167C7, labeled with digoxigenin-11-dUTP, and revealed by FITC-labeled mouse antidigoxigenin antibody (green). Although separate green and red signals indicate the rearrangement of CREB1 region on chromosome 2 (arrows), colocalized signals represent the normal CREB1 region on chromosome 2 (arrowhead). C, only one case showed ATF1 rearrangement. The proximal ATF1 region is hybridized to the BAC probe RP11-189H16, labeled with digoxigenin-11-dUTP, and revealed by FITC-labeled mouse antidigoxigenin antibody (green), whereas the distal ATF1 region is hybridized to the BAC probe RP11-407N8, labeled with biotin-11-dUTP, and revealed by Cy3-labeled streptavidin (red). Rearrangement of the ATF1 region on chromosome 12 is indicated by the presence of separate red and green signals (arrows), whereas colocalized signals represent normal ATF1 region on chromosome 12 (arrowhead).
Volume 27, Issue 1
