Article Figures & Data
Figures
- Fig. 1.
Treatment schedule. Treatment for all treatment groups was started at 15 wk of age. Vehicle control (PBS) or 5-aza injections were administered thrice a week for 10 wk for all groups. Mice were either euthanized at 24 wk or allowed to survive until death. For every TRAMP treatment group, there was a wild-type (WT) control group.
- Fig. 2.
Weight of the prostate (dorsal, lateral, and ventral lobe) at 24 wk and survival. *, P = 0.017 using the Mann-Whitney test between 5-aza survival and PBS survival. Bar, median for each group.
- Fig. 3.
Representative H&E staining of TRAMP tumors from all treatment groups at 24 wk. A, poorly differentiated adenocarcinoma of the prostate from a PBS-treated animal (×20). B, prostatic intraepithelial neoplasia in a 5-aza–treated animal (×60). C, normal prostate from a combined treatment mouse (×60). D, poorly differentiated adenocarcinoma from the castration group (×20). Bar, 100 μm. E and F, metastatic deposits of prostate cancer in the brain.
- Fig. 4.
Treatment with castration + 5-aza significantly increases survival of TRAMP mice. Treatment duration is indicated. A, Kaplan-Meier analysis for PBS and combined treatment. B, Kaplan-Meier analysis for castration and combined treatment. C, Kaplan-Meier analysis for 5-aza and combined treatment. D, Kaplan-Meier analysis of all treatment groups.
Tables
- Table 1.
Effect of treatments on histologic distribution of lesions in TRAMP mice at 24 wks
Treatment group n Histology P LN Mets (%) P Normal PIN WD MD PD Castration + 5-aza 19 58 5 0 0 37 21 PBS 21 0 14 14 5 67 <0.0001* 43 0.19† 5-Aza 22 14 41 5 0 41 0.007‡ 18 0.10§ Castration 19 30 0 0 0 70 0.19∥ 37 0.29¶ Abbreviations: PIN, prostatic intraepithelial neoplasia; WD, well-differentiated prostate cancer; MD, moderately differentiated prostate cancer; PD, poorly-differentiated prostate cancer; LN, lymph node; Mets, metastasis.
↵* Fisher's exact test comparing the frequency of normal pathology between castration + 5-aza and PBS groups.
↵† Fisher's exact test comparing the frequency of lymph node metastasis between castration + 5-aza and PBS groups.
↵‡ Fisher's exact test comparing the frequency of normal pathology between castration + 5-aza and 5-aza groups.
↵§ Fisher's exact test comparing the frequency of lymph node metastasis between 5-aza and PBS groups.
↵∥ Fisher's exact test comparing the frequency of normal pathology between castration + 5-aza and castration groups.
↵¶ Fisher's exact test comparing the frequency of lymph node metastasis between castration and 5-aza groups.
Volume 13, Issue 7
