Article Figures & Data
Figures
- Fig. 1.
A, the location of bacterial artificial chromosome probes used in the FISH assay on chr 21q22. Arrows, the chromosomal location and transcriptional direction of the genes; ovals, the probes in the colors seen in the FISH assay. B, three-color FISH analysis of a TMPRSS2:ERG fusion–positive case. The interstitial deletion is indicated by loss of the blue signal, and a yellowish signal indicates fusion (arrow). C, TMPRSS2:ERG fusion–negative specimen.
- Fig. 2.
Kaplan-Meier analyses of progression-free survival in endocrine-treated patients. A, TMPRSS2:ERG fusion–positive and TMPRSS2:ERG fusion–negative patients had no statistically significant difference in progression-free survival. B, SPINK1 expression–positive patients had significantly worse progression-free survival than SPINK1 expression–negative patients. Relative risk (RR) with 95% confidence interval according to Cox univariate analysis and P-value according to Mantel-Cox test are given.
- Fig. 3.
An example of SPINK1-positive (A) and SPINK1-negative (B) staining in prostate cancer biopsy specimens.
Tables
- Table 1.
Association of clinicopathologic variables with TMPRSS2:ERG fusion and SPINK1 expression
Variable TMPRSS2:ERG fusion according to FISH (%) P SPINK1 expression according to immunohistochemistry (%)* P No fusion Fusion Negative Positive All samples 117 (66) 61 (34) 165 (89) 21 (11) Gleason score, n (%)† <7 34 (76) 11 (24) 41 (85) 7 (15) 7 34 (65) 18 (35) 50 (93) 4 (7) >7 45 (60) 30 (40) 0.2206 68 (87) 10 (13) 0.4849 T stage, n (%)† T1-2 74 (70) 31 (30) 99 (91) 10 (9) T3-4 43 (60) 30 (40) 0.1096 66 (86) 11 (14) 0.2780 M stage, n (%)‡ M0 42 (66) 22 (34) 57 (88) 8 (12) M1 13 (56) 10 (44) 0.4593 8 (67) 4 (33) 0.4391 PSA ng/mL (mean ± SD)§ 206 ± 1092 78 ± 216 0.3641 105 ± 429 63 ± 104 0.6528 Ki-67% (mean ± SD)∥ 6.6 ± 5.3 9.8 ± 6.9 0.0010 7.6 ± 6.1 7.8 ± 5.1 0.6618 Age (mean ± SD)§ 74.3 ± 7.2 71.9 ± 5.9 0.0243 73.8 ± 6.8 71.6 ± 8.4 0.1728 Tumor area % (mean ± SD)¶ 24.3 ± 24.2 37.1 ± 29.9 0.0059 28.6 ± 26.6 33.7 ± 31.1 0.4252 - Table 2.
Multivariate analyses of the prognostic markers
Variable RR (95% CI) P SPINK1* 2.3 (1.1-4.6) 0.038 PSA (>20 vs ≤20 ng/mL) 2.5 (1.4-4.3) 0.000 Gleason score (>7 vs 7 vs <7) 2.3 (1.4-3.0) 0.000 T stage (3+4 vs 1+2) Nonsignificance Ki-67 staining (>10% vs >5%-10% vs ≤5%) Nonsignificance Abbreviations: RR, relative risk; 95% CI, 95% confidence interval.
↵*SPINK1 expression classified as positive when the intensity was 3 and the surface area stained was >10%.
- Table 3.
Association between TMPRSS2:ERG fusion and SPINK1 expression
SPINK1 expression– negative (%) SPINK1 expression– positive (%) Total (%) Fusion-negative 98 (89) 12 (11) 110 (100) Fusion-positive 53 (88) 7 (12) 60 (100) Total 151 19 170 NOTE: P = 1.000; Fisher's exact test.
Supplementary Data
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Volume 16, Issue 10
