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Poster Presentations - Proffered Abstracts

Abstract B61: Encyclopedic noninvasive liquid biopsies for differential diagnosis in prostate cancer

Darshana A. Patil, Dadasaheb Akolkar, Revati Patil, Pooja Fulmali, Pradip Fulmali, Akshay Ainwale, Navin Srivastava, Vineet Datta, Cynthe Sims, Ajay Srinivasan and Rajan Datar
Darshana A. Patil
Datar Cancer Genetics Limited, Nasik, Maharashtra, India.
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Dadasaheb Akolkar
Datar Cancer Genetics Limited, Nasik, Maharashtra, India.
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Revati Patil
Datar Cancer Genetics Limited, Nasik, Maharashtra, India.
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Pooja Fulmali
Datar Cancer Genetics Limited, Nasik, Maharashtra, India.
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Pradip Fulmali
Datar Cancer Genetics Limited, Nasik, Maharashtra, India.
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Akshay Ainwale
Datar Cancer Genetics Limited, Nasik, Maharashtra, India.
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Navin Srivastava
Datar Cancer Genetics Limited, Nasik, Maharashtra, India.
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Vineet Datta
Datar Cancer Genetics Limited, Nasik, Maharashtra, India.
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Cynthe Sims
Datar Cancer Genetics Limited, Nasik, Maharashtra, India.
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Ajay Srinivasan
Datar Cancer Genetics Limited, Nasik, Maharashtra, India.
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Rajan Datar
Datar Cancer Genetics Limited, Nasik, Maharashtra, India.
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DOI: 10.1158/1557-3265.LiqBiop20-B61 Published June 2020
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Abstracts: AACR Special Conference on Advances in Liquid Biopsies; January 13-16, 2020; Miami, FL

Abstract

Differential diagnosis of prostatic adenocarcinomas is based on histopathologic analysis of tumor tissue obtained by transrectal ultrasound (TRUS)-guided biopsies. However, such invasive biopsies are associated with pain, procedural expenses, sequelae, and risk of complications. Though immunocytochemistry (ICC) profiling of circulating tumor cells (CTCs, defined as EpCAM+, panCK+, CD45-) has been previously attempted for diagnosis in some cancers, it has been unviable due to limited yields of CTCs. For the first time we used enrichment of CTCs that permit meaningful ICC profiling for diagnosis of prostate cancers in suspected cases as contradistinguished from cases of benign enlargement of prostate (BEP). We obtained 15 mL of venous blood draw from 650 individuals, of whom 65 had a confirmed diagnosis of either prostate adenocarcinoma and 585 were diagnosed with benign prostate hyperplasia. CTCs were enriched from PBMCs fraction using an epigenetically acting enrichment process that is lethal towards normal (nonmalignant) cells but confers survival privilege on apoptosis-resistant CTCs. Harvested CTCs were confirmed by immunostaining for EpCAM and pan-CK. ICC profiling of these CTCs was also performed with organ-specific markers PSMA and AMACR. Viable CTCs could be obtained from 58 samples (89.2%) out of 65 confirmed prostate cancer cases. Among the 40 samples that were characterized by deep ICC profiling, all samples (100%) were positive for PSMA and AMACR. Among the benign cases, CTCs were observed in 10 samples (1.7%), of which all 10 were positive for PSMA but negative for AMACR. These patients are being followed up. Possibility of other cancers is not ruled out in these patients. Our results show that ICC-based characterization of CTCs can provide necessary diagnostic information noninvasively to substitute for conventional procedures dependent on tissue extraction.

Citation Format: Darshana A. Patil, Dadasaheb Akolkar, Revati Patil, Pooja Fulmali, Pradip Fulmali, Akshay Ainwale, Navin Srivastava, Vineet Datta, Cynthe Sims, Ajay Srinivasan, Rajan Datar. Encyclopedic noninvasive liquid biopsies for differential diagnosis in prostate cancer [abstract]. In: Proceedings of the AACR Special Conference on Advances in Liquid Biopsies; Jan 13-16, 2020; Miami, FL. Philadelphia (PA): AACR; Clin Cancer Res 2020;26(11_Suppl):Abstract nr B61.

  • ©2020 American Association for Cancer Research.
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Clinical Cancer Research: 26 (11 Supplement)
June 2020
Volume 26, Issue 11 Supplement
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Abstract B61: Encyclopedic noninvasive liquid biopsies for differential diagnosis in prostate cancer
Darshana A. Patil, Dadasaheb Akolkar, Revati Patil, Pooja Fulmali, Pradip Fulmali, Akshay Ainwale, Navin Srivastava, Vineet Datta, Cynthe Sims, Ajay Srinivasan and Rajan Datar
Clin Cancer Res June 1 2020 (26) (11 Supplement) B61; DOI: 10.1158/1557-3265.LiqBiop20-B61

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Abstract B61: Encyclopedic noninvasive liquid biopsies for differential diagnosis in prostate cancer
Darshana A. Patil, Dadasaheb Akolkar, Revati Patil, Pooja Fulmali, Pradip Fulmali, Akshay Ainwale, Navin Srivastava, Vineet Datta, Cynthe Sims, Ajay Srinivasan and Rajan Datar
Clin Cancer Res June 1 2020 (26) (11 Supplement) B61; DOI: 10.1158/1557-3265.LiqBiop20-B61
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  • Abstract PO-031: Outcomes of COVID-19 in patients with lung cancer treated in a tertiary hospital in Madrid
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Proffered Abstracts

  • Abstract PO-031: Outcomes of COVID-19 in patients with lung cancer treated in a tertiary hospital in Madrid
  • Abstract PO-083: Prostate cancer and COVID-19: Impact of hormonal therapy on severe outcomes
  • Abstract PO-085: Sex bias in COVID-19-associated Illness severity and mortality in cancer patients: A systematic review and meta-analysis
Show more Proffered Abstracts
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