Article Figures & Data
Figures
- Fig. 1.
Adenovirus LacZ expression vector (Adex1CALacZ) mediated LacZ expression in MIAPaCa-2 cells. Adex1CALacZ panel, the X-gal staining of MIAPaCa-2 cells 3 days after infection of Adex1CALacZ. Approximately 100% of MIAPaCa-2 cells were stained with X-gal at MOI 30. Adex1w1 panel, X-gal staining of MIAPaCa-2 cells 3 days after infection of control vector Adex1w1. No cells expressed LacZ. It could be confirmed that LacZ gene expression was induced by adenovirus expression vector (Adex1CALacZ) in pancreas cancer MIAPaCa-2 cells.
- Fig. 2.
Northern blot hybridization to identify p16 mRNA expression in MIAPaCa-2 cells induced by AdexCACSp16. A:Lane 1, no virus; Lane 2, control virus (Adex1w1); Lane 3, AdexCACSp16 of MOI 30; Lane 4, AdexCACSp16 of MOI 3. The p16 mRNA expression could not be identified in the lanes with no virus and control virus (Adex1w1), as shown in Lanes 1 and 2. However, a high level of p16 mRNA expression could be detected at the expected position in MIAPaCa-2 to which AdexCACSp16 had been added, as shown in Lanes 3 and 4. B: ethidium bromide stainings of RNA.
- Fig. 3.
The cell proliferation curves of MIAPaCa-2 cells with AdexCACSp16, Adex1w1, and control (no virus). Cell proliferation was significantly suppressed by the p16 expression vector (AdexCACSp16), as compared with the control adenovirus group (Adex1w1). Arrow, time point of adding the vectors. Data are shown as means; bars, SD. *, P < 0.05, AdexCACSp16 compared with Adex1w1.
Volume 5, Issue 12
