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Molecular Oncology, Markers, Clinical Correlates

Expression of Cancer Testis Genes in Human Brain Tumors

Ugur Sahin, Michael Koslowski, Özlem Türeci, Thomas Eberle, Carsten Zwick, Bernd Romeike, Jean-Richard Moringlane, Karl Schwechheimer, Wolfgang Feiden and Michael Pfreundschuh
Ugur Sahin
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Michael Koslowski
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Özlem Türeci
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Thomas Eberle
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Carsten Zwick
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Bernd Romeike
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Jean-Richard Moringlane
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Karl Schwechheimer
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Wolfgang Feiden
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Michael Pfreundschuh
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DOI:  Published October 2000
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  • Fig. 1.
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    Fig. 1.

    RT-PCR for the expression of the CT genes SCP-1, SSX-2, and SSX-4 in human brain tumors. An equal amount of testis RNA was used as a representative positive control. Top, expression of SCP-1 by (from left to right with case numbers as indicated in Tables 3<$REFLINK> and 2<$REFLINK> ) astrocytoma II (case 12 of Table 3<$REFLINK> ), astrocytoma IV (case 20, Table 3<$REFLINK> ), oligodendroglioma (case 3, Table 3<$REFLINK> ), oligoastrocytoma (case 10, Table 3<$REFLINK> ), oligoastrocytoma (case 11, Table 3<$REFLINK> ), astrocytoma IV (case 34, Table 3<$REFLINK> ), meningioma (case 1, Table 2<$REFLINK> ), astrocytoma IV (case 26, Table 3<$REFLINK> ), astrocytoma IV (case 18, Table 3<$REFLINK> ), astrocytoma IV (case 31, Table 3<$REFLINK> ), and testis (T) markers. Middle, expression of SSX-2 by testis (T) markers, oligoastrocytoma (case 10, Table 3<$REFLINK> ), oligodendroglioma (case 5, Table 3<$REFLINK> ), astrocytoma IV (case 20, Table 3<$REFLINK> ), oligodendroglioma (case 3, Table 3<$REFLINK> ), astrocytoma II (case 12, Table 3<$REFLINK> ), astrocytoma IV (case 33, Table 3<$REFLINK> ), oligoastrocytoma (case 8, Table 3<$REFLINK> ), oligodendroglioma (case 6, Table 3<$REFLINK> ), astrocytoma IV (case 19, Table 3<$REFLINK> ), meningioma (case 1, Table 1<$REFLINK> ), oligoastrocytoma (case 11, Table 3<$REFLINK> ), anaplastic meningioma (case 6, Table 1<$REFLINK> ), and oligoastrocytoma (case 11, Table 3<$REFLINK> ). Bottom, expression of SSX-4 by testis (T) markers, astrocytoma IV (case 29, Table 3<$REFLINK> ), astrocytoma II (case 16, Table 2<$REFLINK> ), oligodendroglioma (case 7, Table 3<$REFLINK> ), oligodendroglioma (case 5, Table 3<$REFLINK> ), astrocytoma IV (case 18, Table 3<$REFLINK> ), oligodendroglioma (case 3, Table 3<$REFLINK> ), astrocytoma IV (case 33, Table 3<$REFLINK> ), oligoastrocytoma (case 8, Table 3<$REFLINK> ), astrocytoma II (case 15, Table 3<$REFLINK> ), astrocytoma II (case 13, Table 3<$REFLINK> ), astrocytoma IV (case 22, Table 3<$REFLINK> ), meningioma (case 1, Table 2<$REFLINK> ), oligoastrocytoma (case 11, Table 3<$REFLINK> ), and anaplastic meningioma (case 6, Table 2<$REFLINK> ).

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    Fig. 2.

    Immunohistological detection of HOM-TES-14/SCP-1 in an astrocytoma. The tumor cells, especially those of the gemistocytic type, show a fine granular cytoplasmic staining. Some tumor cells also show or exclusively show nuclear reactivity (>). Immunoperoxidase stain (×180).

Tables

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  • Table 3

    %Expression of cancer testis genes by human brain tumors other than meningiomas

    No.DiagnosisPatient’s codeSSX-1SSX-2SSX-4SCP-1TS85ESO-1MAGE-3
    1Ependymoma215−−−−
    2Pilocytic astrocytomaR59−−−+−−
    3OligodendrogliomaR70−−−−−−−
    4OligodendrogliomaR71−−+−−
    5OligodendrogliomaR90−−+−−
    6OligodendrogliomaR82−−−−−−
    7Oligodendroglioma120−−−−−−
    8OligoastrocytomaR21−−+++−−
    9OligoastrocytomaR28−−+−−
    10OligoastrocytomaR56−−+−+−−
    11Oligoastrocytoma731−+++−−−
    12Astrocytoma IIR42−−−−+−−
    13Astrocytoma II469−−+++
    14Astrocytoma II647−+−−−−−
    15Astrocytoma II677−+−−
    16Astrocytoma II706−−+−−−−
    17Astrocytoma IIIG27−−−−−−
    18Astrocytoma IV46−−−−
    19Astrocytoma IV47−−−−
    20Astrocytoma IV84−−−−
    21Astrocytoma IV85+−−+−−−
    22Astrocytoma IV86−−−−−−−
    23Astrocytoma IV90−+−−−−
    24Astrocytoma IV91−+−−−
    25Astrocytoma IV93−−++
    26Astrocytoma IV121−−+
    27Astrocytoma IV208−+−−−
    28Astrocytoma IV219−−−−−
    29Astrocytoma IV705−−+−−−+
    30Astrocytoma IV718−−−−−−−
    31Astrocytoma IV726−−−+−−−
    32Astrocytoma IVR34−−−+−−
    33Astrocytoma IVR35−−+++−−
    34Astrocytoma IVR79+++++−+
    35Astrocytoma IVR96−−−+−−
    36Astrocytoma IV89−−−+−−−
    37Astrocytoma IV95−−−+−−−
    38Astrocytoma IV210−−−−−−−
    39Astrocytoma IV664−−++−−−
    40Astrocytoma IV317/99−−−+−−−
    41Astrocytoma IV386/99−−−−−−−
    42Astrocytoma IV492/99−−−+−−−
    43Astrocytoma IV611/99−−−−−−−
    44Astrocytoma IV613/99−−−−−−−
    45Astrocytoma IV937/99−−−−−−−
    46Astrocytoma IV1164/99−−−−−−−
    47Astrocytoma IV1254/99−−−−−−−
    48Astrocytoma IV1280/99−−−−−−−
    49Astrocytoma IV1460/99−−−−−−−
    50Astrocytoma IVHA−−++−−−
  • Table 1

    %Expression of cancer testis genes by tissues other than human brain tumors

    TissueSSX-1SSX-2SSX-4SCP-1TS85ESO-1MAGE-3
    Normal human tissues
    Testis+++++++
    Brain
    Frontal cortex−−−−−−−
    White matter−−−−−−−
    Cerebellum−−−−−−−
    Bladder−−−−−−−
    Breast−−−−−−−
    Colon−−−−−−−
    Kidney−−−−−−−
    Liver−−−−−−−
    Lung−−−−−−−
    Lymph node−−−−−−−
    Muscle−−−−−−−
    Ovary−−−−−−−
    Peripheral blood lymphocytes−−−−−−−
    Prostate−−−−−−−
    Rectum−−−−−−−
    Skin−−−−−−−
    Spleen−−−−−−−
    Stomach−−−−−−−
    Tonsil−−−−−−−
    Uterus (endometrium)−−−−−−−
    Malignant human tissues
    Bladder cancer2 /94 /92 /94 /5
    Breast cancer5 /675 /6710 /679 /330 /2510 /337 /51
    Colorectal cancer3 /587 /589 /580 /322 /210 /16
    Endometrial cancer1 /81 /81 /80 /7
    Gastric cancer0 /30 /30 /31 /140 /16
    Head & neck cancer3 /145 /144 /140 /2
    Leiomyosarcoma0 /80 /80 /8
    Leukemia0 /310 /310 /310 /11
    Lung cancer1 /244 /241 /241 /142 /12
    Lymphoma0 /114 /110 /110 /10
    Malignant melanoma10 /3713 /3710 /374 /288 /2223 /6712 /23
    Ovarian cancer0 /120 /126 /120 /32 /8
    Prostatic cancer0 /51 /50 /50 /120 /54 /16
    Renal cancer0 /221 /220 /223 /360 /10
    Seminoma0 /30 /30 /30 /25 /130 /1
    Synovial sarcoma0 /42 /41 /40 /30 /2
    Thyroid cancer0 /60 /60 /60 /52 /5
  • Table 2

    %Expression of cancer testis genes by human meningiomas

    No.DiagnosisPatient’s codeSSX-1SSX-2SSX-4SCP-1TS85ESO-1MAGE-3
    1Meningioma733−−−−−−−
    2Meningioma735−−−−−−
    3Meningioma737−−−−−−−
    4Meningioma740−−−−−−−
    5Meningioma741−−−−−−−
    6Meningioma (anaplasia)695−−−+−−−
    7Meningioma192−−−−
    8Meningioma680−−−−
    9Meningioma1210−−−−
    10Meningioma1612−−−−
    11Meningioma1694−−−+
    12Meningioma63/97−−−−
    13Meningioma99/97−−−−
    14Meningioma173/97−−−−
    15Meningioma239/97−−−−
    16Meningioma312/97−−−−
    17Meningioma447/97−−−−
    18Meningioma1129/97−−−−
    19Meningioma1168/97−−−+
    20Meningioma1214/97−−−−
    21Meningioma1400/97−−−−
    22Meningioma1563/97−−−+
    23Meningioma1879/97−−−−
    24Meningioma040/98−−−+
    25Meningioma094/98−−−−
    26Meningioma101/98−−−−
    27Meningioma102/98−−−−
    28Meningioma137/98−−−−
    29Meningioma229/98−−−−
    30Meningioma294/98−−−−
    31Meningioma367/98−−−−
    32Meningioma678/98−−−−
    33Meningioma689/98−−−+
    34Meningioma786/98−−−−
    35Meningioma797/98−−−−
    36Meningioma928/98−−−+
    37Meningioma1214/98−−−−
    38Meningioma1510/98−−−−
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October 2000
Volume 6, Issue 10
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Expression of Cancer Testis Genes in Human Brain Tumors
Ugur Sahin, Michael Koslowski, Özlem Türeci, Thomas Eberle, Carsten Zwick, Bernd Romeike, Jean-Richard Moringlane, Karl Schwechheimer, Wolfgang Feiden and Michael Pfreundschuh
Clin Cancer Res October 1 2000 (6) (10) 3916-3922;

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Expression of Cancer Testis Genes in Human Brain Tumors
Ugur Sahin, Michael Koslowski, Özlem Türeci, Thomas Eberle, Carsten Zwick, Bernd Romeike, Jean-Richard Moringlane, Karl Schwechheimer, Wolfgang Feiden and Michael Pfreundschuh
Clin Cancer Res October 1 2000 (6) (10) 3916-3922;
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