Article Figures & Data
Figures
- Fig. 1.
PAX6 expression in glioma cell lines and chromosome 10 hybrid clones measured by real-time quantitative RT-PCR. A, relative expression level of PAX6 gene normalized by enolase-α (▪) or GAPDH (□), and the value of 1 was assigned for U251. N10.5.6, N10.6, and N10.8 are U251-chromosomal 10 hybrid clones. NHA is a normal astrocytic cell line. The tumorigenicity of each cell line in nu/nu mice (LT, low tumorigenic) is from Ke et al. (14) and Ishii et al. (28) . B, comparison of relative PAX6 expression level in glioma cells grouped into none- and low- (NT/LT) tumorigenic and HT cells. The P was from a two-sample unequal variance t test. The position of a bar and a vertical line for each class indicates the mean and SD, respectively.
- Fig. 2.
Analysis of the relative levels of PAX6, PTEN, VEGF, and EGFR gene expression by real-time quantitative RT-PCR in 45 AA, 42 GBM, and 7 samples of adjacent normal tissue, and normalized by β-actin. The Y axis is a log 10-transformed PAX6:β-actin ratio. The position of a bar and a vertical line for each class indicate the mean and SD, respectively. The statistical significance between AAs and GBMs is shown by Ps.
- Fig. 3.
Kaplan-Meier survival curves for 86 patients with astrocytic malignant gliomas above and below the cutpoint of the β-actin normalized PAX6 gene expression value derived from optimal dichotomization.
- Fig. 4.
Kaplan-Meier survival curves for the same set of patients as in Fig. 3<$REFLINK> based on β-actin normalized expression values of PAX6 and PTEN genes derived from recursive partitioning analysis.
Tables
- Table 1
Median expression levels normalized by β-actin
Genes Normal (n = 7) AA (n = 45) GBM (n = 42) PAX6 0.018 0.019 0.006 PTEN 0.012 0.010 0.004 EGFR 0.007 0.013 0.027 VEGF 0.029 0.021 0.086 - Table 2
Comparison of relative PAX6 expression levels in the same patients
Patient # Tissue PAX6/β-actin Normal/tumor (AA/GBM) 1 Normal 0.071 11.2 GBM 0.006 2 Normal 0.027 9.0 GBM 0.003 3 Normal 0.012 1.9 GBM 0.006 4 Normal 0.010 4.0 GBM 0.003 5 Normal 0.013 2.5 GBM 0.005 6 Normal 0.081 1.9 AA 0.042 7 Normal 0.018 1.0 AA 0.019 8 AA 0.032 31.1 GBM 0.001 - Table 3
P from Cox proportional hazards regression analyses using dichotomized cutpoints
Gene Univariatea 4 variatesb 7 variatesc PAX6 0.0002 0.0074 0.0006 PTEN <0.0001 0.0011 0.0002 VEGF <0.0001 0.0065 0.0006 EGFR 0.021 0.12 0.80 a Each gene analyzed individually.
b Same as for a, but also adjusting for age, histology, and recurrence status.
c All four genes included in a single model with age, histology, and recurrence status.
- Table 4
Proportion of variation in survival explained by the models
Model Clinical variates Clinical variates + each gene 7 variates PAX6 PTEN VEGF EGFR Proportion 30% 36% 39% 37% 32% 55%
Volume 9, Issue 9
