RT Journal Article
SR Electronic
T1 Gene Expression Differences Associated with Human Papillomavirus Status in Head and Neck Squamous Cell Carcinoma
JF Clinical Cancer Research
JO Clin Cancer Res
FD American Association for Cancer Research
SP 701
OP 709
DO 10.1158/1078-0432.CCR-05-2017
VO 12
IS 3
A1 Slebos, Robbert J.C.
A1 Yi, Yajun
A1 Ely, Kim
A1 Carter, Jesse
A1 Evjen, Amy
A1 Zhang, Xueqiong
A1 Shyr, Yu
A1 Murphy, Barbara M.
A1 Cmelak, Anthony J.
A1 Burkey, Brian B.
A1 Netterville, James L.
A1 Levy, Shawn
A1 Yarbrough, Wendell G.
A1 Chung, Christine H.
YR 2006
UL http://clincancerres.aacrjournals.org/content/12/3/701.abstract
AB Human papillomavirus (HPV) is associated with a subset of head and neck squamous cell carcinoma (HNSCC). Between 15% and 35% of HNSCCs harbor HPV DNA. Demographic and exposure differences between HPV-positive (HPV+) and negative (HPV−) HNSCCs suggest that HPV+ tumors may constitute a subclass with different biology, whereas clinical differences have also been observed. Gene expression profiles of HPV+ and HPV− tumors were compared with further exploration of the biological effect of HPV in HNSCC. Thirty-six HNSCC tumors were analyzed using Affymetrix Human 133U Plus 2.0 GeneChip and for HPV by PCR and real-time PCR. Eight of 36 (22%) tumors were positive for HPV subtype 16. Statistical analysis using Significance Analysis of Microarrays based on HPV status as a supervising variable resulted in a list of 91 genes that were differentially expressed with statistical significance. Results for a subset of these genes were verified by real-time PCR. Genes highly expressed in HPV+ samples included cell cycle regulators (p16INK4A, p18, and CDC7) and transcription factors (TAF7L, RFC4, RPA2, and TFDP2). The microarray data were also investigated by mapping genes by chromosomal location (DIGMAP). A large number of genes on chromosome 3q24-qter had high levels of expression in HPV+ tumors. Further investigation of differentially expressed genes may reveal the unique pathways in HPV+ tumors that may explain the different natural history and biological properties of these tumors. These properties may be exploited as a target of novel therapeutic agents in HNSCC treatment.