RT Journal Article
SR Electronic
T1 Rapid (“Warm”) Autopsy Study for Procurement of Metastatic Prostate Cancer
JF Clinical Cancer Research
JO Clin Cancer Res
FD American Association for Cancer Research
SP 1038
OP 1045
VO 6
IS 3
A1 Rubin, Mark A.
A1 Putzi, Mathew
A1 Mucci, Neil
A1 Smith, David C.
A1 Wojno, Kirk
A1 Korenchuk, Susan
A1 Pienta, Kenneth J.
YR 2000
UL http://clincancerres.aacrjournals.org/content/6/3/1038.abstract
AB In this report, we describe the distribution of metastases from 14 patients who had hormone-refractory adenocarcinoma of the prostate and agreed while alive to undergo directed autopsies after their deaths. These autopsies were undertaken specifically to document the distribution of metastases, characterize tumors phenotypically and immunohistochemically, harvest fresh and snap frozen tumor and normal control tissues suitable for molecular examination, and establish cell lines via passages through generations of severe combined immunodeficient and athymic mice. Achievement of these goals was obtained through the development of a multidisciplinary team approach. Team members included a medical oncologist, pathologists, urologists, and researchers. The autopsy and tissue procurement teams were available on a round-the-clock basis. The tissues harvested from these autopsies yielded high-quality tumor samples, as evidenced by excellent preservation seen by light microscopy, strong prostate-specific antigen immunostaining, and the successful development of xenografts. The development and expansion of this program represent a valuable resource for molecular and clinical researchers.