Table 6

PC12 transcription pattern using a microarrays filter system

In these experiments, the pattern of gene expression of untreated cells (ctr) was compared with 1 ng/ml NGF, 1 mm ALCAR, or 1 mm ALCAR plus 1 ng/ml NGF of 2-h treated PC12 cells.

GenBank accession no.Protein/geneModulation of gene expression by treatmentsa
1 mm ALCAR1 ng/ml NGF1 ng/ml NGF + 1 mm ALCAR
M20035;prothymosin-alpha (PTMA)+/+
D10754proteasome delta subunit precursor; macropain delta; multicatalytic endopeptidase complex delta; proteasome subunit Y; proteasome subunit 5; PSMB6+/+
M86389heat shock 27-kDa protein (HSP27)//
J03752microsomal glutathione S-transferase (GST12; MGST1)+/+
U43175ATPase, subunit F, vacuolar (vatf)+//
K0293340S ribosomal protein S17 (RPS17)++/
D44495apurinic/apyrimidinic endonuclease (AP endonuclease; APEX; APEN)+//
D10864Id-3; DNA-binding protein inhibitor; HLH protein/++
X06942A-raf proto-oncogene/+/
D12771fibroblast ADP/ATP carrier protein; ADP/ATP translocase 2; adenine nucleotide translocator 2 (ANT2)/+/
M60525VGF8A protein precursor/+++
D38222Tyrosine phosphatase-like protein; negative regulator of PTPases in neuronal tissues, RAT PHEOCHROMOCYTOMA-DERIVED PROTEIN TYROSINE PHOSPHATASE-LIKE PROTEIN (EC 3.1.3.48)/++
D10862DNA-binding protein inhibitor ID1//+
D10863Id-2; DNA-binding protein inhibitor; HLH protein//+
X16956 + U26663microglobulin; beta-2-microglobulin + prostaglandin receptor F2a//+
D13123ATP synthase lipid-binding protein P1 precursor; ATPase protein 9; ATP5G1//+
X64827cytochrome c oxidase, subunit VIIIh//+
D10021mitochondrial ATP synthase D subunit; ATP5H//+
M19044mitochondrial ATP synthase beta subunit precursor (ATP5B)//+
X51707S19; 40S ribosomal protein S19//+
M84716Fte-1; putative v-fos transformation effector protein; yeast mitochondrial protein import homolog; 40S ribosomal protein S3A; RPS3A//+
X06832chromogranin A//+
M18416; J04154early growth response protein 1 (EGR1); nerve growth factor-induced protein A (NGFI-A)//++
L07578Casein kinase I delta; CKId; 49-kDa isoform//+
D1761514-3-3 protein zeta/delta; PKC inhibitor protein-1; KCIP-1; mitochondrial import stimulation factor S1 subunit//+
L12384ADP-ribosylation factor 5 (ARF5)//+
  • a Symbols indicate how many fold the particular gene was modulated by treatment: / = not modulated; + = up-regulated >2-fold; ++ = up-regulated >5-fold.