Table 1

Technical conditions of immunostaining

AntigenM/PaAntibody cloneSourceRetrievingDilution
P53MDO7Dako, Glostrup, DenmarkMW 10 min. (pH 6)1/20
P16INK4PP16-C20Santa Cruz Biotechnology, Santa Cruz, CANo1/200
Cyclin D1MDCS-6Calbiochem, Cambridge, United KingdomMW 10 min. (pH 6)1/4000 kit tyramine CSA; Dako
Cyclin EM13 A3Novocastra L., Newcastle, United KingdomMW M. S. Unmasker (pH 8)1/50
Bcl2M124DakoMW 10 min. (pH 6)1/25
BaxMN19Santa Cruz BiotechnologyMW 10 min. (pH 6)1/200
  • a M, mouse monoclonal antibody; P, rabbit polyclonal antibody; MW, microwave heating.