Table 1

Characteristics of the cases of DLBCL investigated for the t(14;18) by FISH

A total of 137 de novo DLBCLs were classified according to their GC status defined by expression of BCL6 protein and CD10. BCL2 protein expression and the t(14;18), as detected by interphase FISH on paraffin-extracted nuclei, were significantly associated with a GC phenotype.

Overall n = 137BCL2/t(14;18) statusχ2 significance
BCL2−, t(14;18)−a n = 79 (58%)BCL2+, t(14;18)− n = 40 (29%)BCL2+, t(14;18)+ n = 18 (13%)
GC statusP < 0.001
 GC type4719 (40%)14 (30%)14 (30%)
 Non-GC8959 (66%)26 (29%)4 (5%)
 N/Ab11
IPIP = 0.8
 Low (0–1)5332 (60%)16 (30%)5 (10%)
 Intermediate (2–3)5731 (54%)16 (28%)10 (18%)
 High (4–5)85 (62%)2 (25%)1 (13%)
 N/A191162
Bone marrowP = 0.03
 Normal7549 (65%)18 (24%)8 (11%)
 Involvedc144 (29%)6 (42%)4 (29%)
 Not done48
  • a The t(14;18) was not detected in any of the BCL2 protein negative cases.

  • b N/A, not applicable.

  • c Ten of 14 cases were marrow infiltration with DLBCL, and the remaining 4 cases had low-level involvement with FL. There was no association between the type of marrow involvement and the presence of BCL2 abnormalities (data not shown).