Table 1

Sequences of AS ODNs and their target site on XIAP mRNA

OligonucleotideSequenceaPosition (nucleotide no.)b
F3 ASATCTTCTCTTGAAAATAGG17–35
F3 RPGGATAAAAGTTCTCTTCTA
G4 ASGCTGAGTCTCCATATTGCC251–269
G4 SCGGCTCTTTGCCCACTGAAT
C5 ASACCATTCTGGATACCAGAA354–372
C5 RPAAGACCATAGGTCTTACCA
AB6 ASGGGTTCCTCGGGTATATGG470–488
AB6 RPGGTATATGGCGTCCTTGGG
DE4 ASGGTATCTCCTTCACCAGTA166–184
DE4 RPATGACCACTTCCTCTATGG
D7 ASGATTCACTTCGAATATTAA719–737
D7 RPAATTATAACGTTCACTTAG
  • a Chimeric (2 × 2 PS/PO) and fully phosphorothioated AS ODNs (PS-ODN) have the same sequence, with the exception of the substitution of a uracyl base (U) for a thymidine base (T) in the RNA flanking regions (underlined sequences). Differences in chemical structures were described in “Materials and Methods.” Corresponding control oligonucleotides are shown either as RP or as SC sequences.

  • b The 2540-bp cDNA (partial mRNA, GenBank accession no. U45880) was used for numbering.