Table 1.

Relative mRNA levels (× 10–4) in various genes in different subcutaneous prostate tumor cell lines compared with the levels in glyceraldehyde-3-phosphate dehydrogenase

GeneTumor cell line
MDA prostate cancer 118bPC-3MDA prostate cancer 2b
FGF9863 ± 121 ⇑3 ± 0.2Undetectable
BMP41331 ± 190 ⇑19 ± 4Undetectable
ITF22783 ± 197 ⇑46 ± 6Undetectable
NrCAM3581 ± 275 ⇑39 ± 1241 ± 5
SOX27904 ± 121 ⇑59 ± 1024 ± 2
OCT466 ± 662 ± 11158 ± 31
ARUndetectable7 ± 22870 ± 820
Cyclin D163 ± 27505 ± 193823 ± 115
c-Myc424 ± 571500 ± 4791552 ± 336
  • NOTE: For each gene, data from 3 independent reactions were used to calculate the means and SDs shown. If no value in the linear range of PCR cycles of a specific RNA was detected, probably because little or no mRNA was expressed in that particular tumor sample, it was considered undetectable. Primers were human specific, and these were tested by mRNA isolated from primary mouse osteoblasts and PC-3 cells grown in vitro. Arrows indicate the genes that are upregulated in MDA prostate cancer 118b relative to those in both PC-3 and MDA prostate cancer 2b cells.