Table 3.

Summary of functional assessment of mutations

WT-Cx47G146SaG183CP381SH409Y
LEC spreadb,c1.4 ± 0.142.0 ± 0.131.4 ± 0.182.26 ± 0.17d0.07 ± 0.07d
Electrical couplingb,f35.9 ± 8.932.4 ± 8.668.6 ± 11.3e48.2 ± 8.646.4 ± 12.9
Wound assayb,g0.72 ± 0.010.62 ± 0.03e0.68 ± 0.160.70 ± 0.030.73 ± 0.02
  • aG146S mutation also found in patients with primary lymphedema (16).

  • bMean ± SEM.

  • cDye spread to tiers of LECs after 2 minutes.

  • dIndicates significance determined by Mann–Whitney test.

  • eIndicates significantly different than WT-hCx47 at P < 0.05, 2-tailed Student t test.

  • fIn nS.

  • gExpressed as fraction of original wound in HeLa cell monolayer after 24 hours.