Table 2.

Genes downregulated in cancer stroma, which are putative targets of miR-17-92a and/or miR-25-106b clusters

Gene symbolGenbank accessionDescriptionFold ChangeaQ (%)amiR-17/20a/106b/93 targetbmiR-18a targetbmiR-19a/19b targetbmiR-92a/25 targetb
KEGG 04061: Cytokine–cytokine receptor interaction (chemokines, hematopoietins, PDGF family, TNF family, and TGFβ family)
IL10RANM_001558Homo sapiens interleukin 10 receptor, α (IL10RA)0.24920.0000
TNFRSF17NM_001192Homo sapiens TNF receptor superfamily, member 17 (TNFRSF17)0.13130.0000
LIFRNM_002310Homo sapiens leukemia inhibitory factor receptor alpha (LIFR)0.15630.0000
CXCL12NM_199168Homo sapiens chemokine (C-X-C motif) ligand 12 (stromal cell–derived factor 1; CXCL12), transcript variant 10.12180.0000
BMP2NM_001200Homo sapiens bone morphogenetic protein 2 (BMP2)0.17340.0748
CNTFRNM_147164Homo sapiens ciliary neurotrophic factor receptor (CNTFR), transcript variant 10.21420.0748
IL6RNM_000565Homo sapiens interleukin 6 receptor (IL6R), transcript variant 10.26940.0748
PDGFRABC015186Homo sapiens platelet-derived growth factor receptor, α polypeptide, mRNA (cDNA clone IMAGE: 4043984), complete cds.0.22470.1256
TNFRSF13BNM_012452Homo sapiens TNF receptor superfamily, member 13B (TNFRSF13B)0.20330.1256
XCL1NM_002995Homo sapiens chemokine (C motif) ligand 1 (XCL1)0.16760.1256
CTF1NM_001330Homo sapiens cardiotrophin 1 (CTF1)0.31780.2025
XCL2NM_003175Homo sapiens chemokine (C motif) ligand 2 (XCL2)0.20280.2025
CCR9NM_006641Homo sapiens chemokine (C-C motif) receptor 9 (CCR9), transcript variant B0.25140.2025
TGFBR2NM_003242Homo sapiens TGF, β receptor II (70/80 kDa) (TGFBR2), transcript variant 20.38190.3097
CCL16NM_004590Homo sapiens chemokine (C-C motif) ligand 16 (CCL16)0.35020.4833
IFNA2NM_000605Homo sapiens IFN, α 2 (IFNA2)0.41670.7799
GHRNM_000163Homo sapiens growth hormone receptor (GHR)0.35380.7799
IFNW1NM_002177Homo sapiens IFN, omega 1 (IFNW1)0.39260.7799
IL15NM_172174Homo sapiens interleukin 15 (IL15), transcript variant 10.43310.7799
LEPNM_000230Homo sapiens leptin (obesity homolog, mouse; LEP)0.35910.7799
BMPR1BNM_001203Homo sapiens bone morphogenetic protein receptor, type IB (BMPR1B)0.42511.2419
FASLGNM_000639Homo sapiens Fas ligand (TNF superfamily, member 6; FASLG)0.42841.2419
CCL13NM_005408Homo sapiens chemokine (C-C motif) ligand 13 (CCL13)0.24041.2419
CCL5NM_002985Homo sapiens chemokine (C-C motif) ligand 5 (CCL5)0.38391.2419
IL9RNM_176786Homo sapiens interleukin 9 receptor (IL9R), transcript variant 20.48041.2419
IL18R1NM_003855Homo sapiens interleukin 18 receptor 1 (IL18R1)0.46671.2419
IL29NM_172140Homo sapiens interleukin 29 (IFN, lambda 1; IL29)0.41562.2195
TNFRSF19NM_018647Homo sapiens TNF receptor superfamily, member 19 (TNFRSF19), transcript variant 10.48432.2195
TSLPNM_033035Homo sapiens thymic stromal lymphopoietin (TSLP), transcript variant 10.38412.2195
IL25NM_022789Homo sapiens interleukin 25 (IL25), transcript variant 10.47752.2195
KEGG 04340: Hedgehog signaling pathway
WNT10ANM_025216Homo sapiens wingless type MMTV integration site family, member 10A (WNT10A)0.12640.0000
BMP2NM_001200Homo sapiens bone morphogenetic protein 2 (BMP2)0.17340.0748
BMP6NM_001718Homo sapiens bone morphogenetic protein 6 (BMP6)0.20970.2025
BMP5NM_021073Homo sapiens bone morphogenetic protein 5 (BMP5)0.17470.3097
WNT9ANM_003395Homo sapiens wingless-type MMTV integration site family, member 9A (WNT9A)0.38300.7799
WNT1NM_005430Homo sapiens wingless-type MMTV integration site family, member 1 (WNT1)0.42260.7799
CSNK1A1AF447582Homo sapiens HLCDGP1 mRNA, complete cds.0.49521.2419
WNT7BNM_058238Homo sapiens wingless-type MMTV integration site family, member 7B (WNT7B)0.47041.2419
BMP8ANM_181809Homo sapiens bone morphogenetic protein 8a (BMP8A)0.35962.2195
HHIPAK074711Homo sapiens cDNA FLJ90230 fis, clone NT2RM20004100.44943.4908
KEGG 05200: Pathways in cancer
CASP8NM_033356Homo sapiens caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant C0.13130.0000
COL4A4NM_000092Homo sapiens collagen, type IV, alpha 4 (COL4A4)0.07900.0000
WNT10ANM_025216Homo sapiens wingless-type MMTV integration site family, member 10A (WNT10A)0.12640.0000
BCL2NM_000633Homo sapiens B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant α0.24900.0353
RASSF5NM_182664Homo sapiens Ras association (RalGDS/AF-6) domain family 5 (RASSF5), transcript variant 20.20310.0353
BMP2NM_001200Homo sapiens bone morphogenetic protein 2 (BMP2)0.17340.0748
VHLAF088066Homo sapiens full-length insert cDNA clone ZD86C03.0.31460.0748
PDGFRABC015186Homo sapiens platelet-derived growth factor receptor, α polypeptide, mRNA (cDNA clone IMAGE:4043984), complete cds0.22470.1256
IGF1NM_000618Homo sapiens insulin-like growth factor 1 (somatomedin C; IGF1)0.29910.2025
ETS1NM_005238Homo sapiens v-ets erythroblastosis virus E26 oncogene homolog 1 (avian; ETS1)0.37620.3097
TGFBR2NM_003242Homo sapiens TGF, β receptor II (70/80 kDa) (TGFBR2), transcript variant 20.38190.3097
NKX3-1NM_006167Homo sapiens NK3 transcription factor related, locus 1 (Drosophila; NKX3-1)0.42310.4833
CBLNM_005188Homo sapiens Cas-Br-M (murine) ecotropic retroviral transforming sequence (CBL)0.44980.4833
STK4BC005231Homo sapiens serine/threonine kinase 4, mRNA (cDNA clone IMAGE:3950315), complete cds.0.40240.4833
SMAD2NM_005901Homo sapiens SMAD family member 2 (SMAD2), transcript variant 10.38460.4833
FGFR2NM_022970Homo sapiens fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte growth factor receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer syndrome, Jackson–Weiss syndrome; FGFR2), transcript variant 2, mRNA0.30380.4833
WNT9ANM_003395Homo sapiens wingless-type MMTV integration site family, member 9A (WNT9A)0.38300.7799
WNT1NM_005430Homo sapiens wingless-type MMTV integration site family, member 1 (WNT1)0.42260.7799
COL4A6NM_033641Homo sapiens collagen, type IV, α 6 (COL4A6), transcript variant B0.19320.7799
PIK3CDNM_005026Homo sapiens phosphoinositide-3-kinase, catalytic, delta polypeptide (PIK3CD)0.45800.7799
DAPK2NM_014326Homo sapiens death-associated protein kinase 2 (DAPK2)0.38100.7799
FGF6NM_020996Homo sapiens fibroblast growth factor 6 (FGF6)0.39130.7799
PLD1NM_002662Homo sapiens phospholipase D1, phosphatidylcholine specific (PLD1)0.46850.7799
FASLGNM_000639Homo sapiens Fas ligand (TNF superfamily, member 6; FASLG)0.42841.2419
RUNX1X90978Homo sapiens mRNA for an acute myeloid leukaemia protein (1,793 bp)0.45191.2419
SMAD3U68019Homo sapiens mad protein homolog (hMAD-3) mRNA, complete cds0.49431.2419
LAMA3NM_000227Homo sapiens laminin, alpha 3 (LAMA3), transcript variant 20.43291.2419
DCCNM_005215Homo sapiens deleted in colorectal carcinoma (DCC)0.43421.2419
WNT7BNM_058238Homo sapiens wingless-type MMTV integration site family, member 7B (WNT7B)0.47041.2419
FGF5NM_004464Homo sapiens fibroblast growth factor 5 (FGF5), transcript variant 10.48232.2195
HHIPAK074711Homo sapiens cDNA FLJ90230 fis, clone NT2RM2000410.0.44943.4908
KEGG 04514: Cell adhesion molecules (CAM)
CNTN2NM_005076Homo sapiens contactin 2 (axonal; CNTN2)0.16590.0748
ALCAMNM_001627Homo sapiens activated leukocyte cell adhesion molecule (ALCAM)0.29750.1256
CD22NM_001771Homo sapiens CD22 molecule (CD22)0.13590.1256
SPNNM_001030288Homo sapiens sialophorin (leukosialin, CD43; SPN), transcript variant 10.31100.2025
CLDN18NM_016369Homo sapiens claudin 18 (CLDN18), transcript variant 10.34000.2025
CNTNAP2NM_014141Homo sapiens contactin-associated protein-like 2 (CNTNAP2)0.30820.3097
CLDN19NM_148960Homo sapiens claudin 19 (CLDN19)0.38400.4833
PTPRCNM_002838Homo sapiens protein tyrosine phosphatase, receptor type, C (PTPRC), transcript variant 10.36290.7799
NRXN1NM_004801Homo sapiens neurexin 1 (NRXN1), transcript variant alpha0.36740.7799
CD8ANM_001768Homo sapiens CD8a molecule (CD8A), transcript variant 10.40331.2419
CLDN16NM_006580Homo sapiens claudin 16 (CLDN16)0.45431.2419
CNTNAP1NM_003632Homo sapiens contactin-associated protein 1 (CNTNAP1)0.47251.2419
NCAM1NM_000615Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 10.42471.2419
CD28NM_006139Homo sapiens CD28 molecule (CD28)0.49972.2195
L1CAMNM_000425Homo sapiens L1 cell adhesion molecule (L1CAM), transcript variant 10.49383.4908
WNT9ANM_003395Homo sapiens wingless-type MMTV integration site family, member 9A (WNT9A)0.38300.7799
WNT1NM_005430Homo sapiens wingless-type MMTV integration site family, member 1 (WNT1)0.42260.7799
COL4A6NM_033641Homo sapiens collagen, type IV, alpha 6 (COL4A6), transcript variant B0.19320.7799
PIK3CDNM_005026Homo sapiens phosphoinositide-3-kinase, catalytic, delta polypeptide (PIK3CD)0.45800.7799
DAPK2NM_014326Homo sapiens death-associated protein kinase 2 (DAPK2)0.38100.7799
FGF6NM_020996Homo sapiens fibroblast growth factor 6 (FGF6)0.39130.7799
PLD1NM_002662Homo sapiens phospholipase D1, phosphatidylcholine-specific (PLD1)0.46850.7799
FASLGNM_000639Homo sapiens Fas ligand (TNF superfamily, member 6; FASLG)0.42841.2419
RUNX1X90978Homo sapiens mRNA for an acute myeloid leukaemia protein (1,793 bp).0.45191.2419
SMAD3U68019Homo sapiens mad protein homolog (hMAD-3)0.49431.2419
LAMA3NM_000227Homo sapiens laminin, alpha 3 (LAMA3), transcript variant 20.43291.2419
DCCNM_005215Homo sapiens deleted in colorectal carcinoma (DCC)0.43421.2419
WNT7BNM_058238Homo sapiens wingless-type MMTV integration site family, member 7B (WNT7B)0.47041.2419
FGF5NM_004464Homo sapiens fibroblast growth factor 5 (FGF5), transcript variant 10.48232.2195
HHIPAK074711Homo sapiens cDNA FLJ90230 fis, clone NT2RM2000410.0.44943.4908

NOTE: KEGG (ref. 18).

  • aFold change < 0.50, Q < 0.05

  • bBased on the computational analysis by TargetScan (ref. 19, 20). Genes which are targets of each miRNA are marked with circle.