Table 3.

EGFR mutation analysis on BAL and pleura fluids (Series B) by Sanger sequencing and NGS

EGFR mutational analysis
CytologySurgical sample
IDSample typeTumor cells (%)Sanger sequencingNGSSanger sequencing
1nBALn.a.Wild-typeWild-type2235_2249del 15bp
2nBALn.a.Wild-type2235_2249del 15bp2235_2249del 15bp
3nBALn.a.Wild-type2240_2257del 18bp2240_2257del 18bp
4nPFn.a.Wild-typeWild-typeT 2573G
5nBALn.a.Wild-typeT 2573GT 2573G
6nPFn.a.Wild-typeWild-type2236_2250del 15bp
7nPFn.a.Wild-typeWild-type2235_2249del 15bp
8nBALn.a.Wild-typeT 2573GT 2573G
9nPFn.a.Wild-type2236_2250del 15bp2236_2250del 15bp
10nPFn.a.Wild-typeWild-type2235_2249del 15bp
11nBALn.a.Wild-typeWild-typeT 2573G
12nBALn.a.Wild-typeWild-typeT 2573G

NOTE: Comparison with EGFR mutations by Sanger sequencing on matched surgical samples.

Abbreviations: ID, identification number; n.a., not assessable; PF, pleural fluids.