Table 2.

EGFR mutation analysis on BAL and pleura fluids (Series A) by Sanger sequencing and NGS

EGFR mutational analysis
CytologySurgical sample
IDSample typeTumor cells (%)Sanger sequencingNGSSanger sequencing
1PF0.3Wild-typeWild-type2236_2250del 15bp
2BAL8Wild-type2235_2249del 15bp2235_2249del 15bp
3BAL5Wild-type2240_2257del 18bp2240_2257del 18bp
4BAL0.3Wild-typeWild-typeT 2573G
5BAL9T 2573GT 2573GT 2573G
6PF72236_2250del 15bp2236_2250del 15bp2236_2250del 15bp
7BAL5Wild-type2235_2249del 15bp2235_2249del 15bp
8BAL0.3Wild-typeT 2573GT 2573G
9BAL4Wild-type2236_2250del 15bp2236_2250del 15bp
10PF72235_2249del 15bp2235_2249del 15bp2235_2249del 15bp
11BAL1Wild-typeWild-typeT 2573G
12BAL7Wild-type2237_2255delinsT 18bp2237_2255delinsT 18bp
2237_2257delinsTCT 18bpa
13BAL2Wild-typeWild-typeT 2573G
14PF1Wild-typeT2582AT2582A
15BAL9Wild-typeT 2573GT 2573G
16BAL5Wild-type2235_2252delinsATT 15bp2235_2252delinsATT15bp
2235_2251delinsAATTC12ba
17PF0.3Wild-typeWild-type2240_2257del 18bp
18BAL82235_2249del 15bp2235_2249del 15bp2235_2249del 15bp
19PF7Wild-typeT 2573GT 2573G
20PF5Wild-typeT 2573GT 2573G
21BAL0.3Wild-type2240_2257del 18bp2240_2257del 18bp
22BAL2Wild-typeT 2573GT 2573G
23BAL0.3Wild-type2235_2249del 15bp2235_2249del 15bp
24PF0.5Wild-type2236_2250del 15bp2236_2250del 15bp
25BAL9Wild-typeT 2573GT 2573G
26PF0.5Wild-typeWild-type2235_2249del 15bp
27BAL2Wild-type2235_2249del 15bp2235_2249del 15bp
28BAL1Wild-typeT 2573GT 2573G
29PF5Wild-type2235_2249del 15bp2235_2249del 15bp
30BAL0.5Wild-type2236_2250del 15bp2236_2250del 15bp
31BAL2Wild type2236_2250del 15bp2236_2250del 15bp
32BAL52240_2257del 18bp2240_2257del 18bp2240_2257del 18bp
33BAL0.3Wild typeWild typeT 2573G
34BAL0.5Wild typeT 2573GT 2573G
35BAL0.3Wild type2237_2255delinsT 18bp2237_2255delinsT 18bp
36BAL0.3Wild type2235_2249del 15bp2235_2249del 15bp

NOTE: Comparison with EGFR mutations by Sanger sequencing on matched surgical samples.

Abbreviations: ID, identification number; PF, pleural fluids.

  • aSubclone of DNA molecules carrying a deletion different from the main one.