Table 1.

Mutational profile of EEC cell lines

Cell lineEGFRKRASNRASHRASPIK3CAPIK3R1PTENPTEN protein
1AN3-CAR557_K561>QR130fsAbsent
2ECC-1L570PV317fs; V290fsAbsent
3EFE-184
4ENT1025A, T1031AN260SK267fsAbsent
5HEC-1-AG12DG1049R
6HEC-1-BG12DG1049R
7HEC-108A331VK6fs; E288fsAbsent
8HEC-116R88QR55_L70>S; R173CAbsent
9HEC-151G12DC420RI33del; Y76fsWeak
10HEC-251M1043IS10N; E299XWeak
11HEC-265H180fs; Q586fsL318fsAbsent
12HEC-50BG12DE468InsGEYDRLYE
13HEC-59R38CK567E; S460fsY46H; R233X; P246L; L265fsAbsent
14HEC-6A289VR108H; C420fsV85fs; V290fsAbsent
15HHUAG12VR88QK164fs; V290fsAbsent
16IshikawaL570PV317fs; V290fsAbsent
17JHUEM-3
18KLE
19MFE-280H1047Y; I391M
20MFE-296I20M; P539RR130Q; N323fs
21NOU-1G12DR38HNo mRNA expressionAbsent
22RL95-2Q61HR386GM134I; R173H; N323fsAbsent
23SNG-IIK6fsAbsent
24SNG-MG12VR88QK164fs; V290fsAbsent

NOTE: Mutations identified in 24 human EEC cell lines using Sequenom OncoCarta Panel v1.0 and Sanger sequencing for PTEN, PIK3CA, and PIK3R1 transcripts (amino acid changes shown). For PTEN protein, see Supplementary Fig. S2B.