Table 6.

Results of massively parallel sequencing of tumors obtained from the six patients in this study.

BRCA1 mutation reads (%)BRCA2 mutation reads (%)TP53 mutation reads (%)
PatientTime of tumor biopsyBiopsy siteBRCA1 mutation (rs number)Wild-type allelesMutant allelesBRCA2 mutation (rs number)Wild-type allelesMutant allelesTP53 mutationWild-type allelesMutant alleles
1DiagnosisRight ovaryc.3005delA/p.N1002fs (80357846)257 (58)183 (42)c.5946delT p.2003 (80359550)17 (89)2 (11)c.949C>T/p.Q317*169 (76)52 (24)
DiagnosisLeft ovaryc.3005delA/p.N1002fs (80357846)79 (24)244 (76)c.949C>T/p.Q317*179 (79)48 (21)
DiagnosisLNc.3005delA/p.N1002fs (80357846)112 (21)426 (79)c.949C>T/p.Q317*178 (77)52 (23)
Post-PARPiLN 1c.3005delA/p.N1002fs (80357846)102 (18)453 (82)c.949C>T/p.Q317*164 (71)66 (29)
Post-PARPiLN 2c.3005delA/p.N1002fs (80357846)88 (17)435 (83)c.949C>T/p.Q317*160 (73)59 (27)
2DiagnosisRight ovaryc.3760_3761insT/p.K1254fs (80357986)87 (42)120 (58)c.659A>G/p.Y220C15 (42)21 (58)
Post-PARPiPelvic sidewall tumorc.3760_3761insT/p.K1254fs (80357986)130 (51)126 (49)c.659A>G/p.Y220C1 (6)16 (94)
3DiagnosisLeft ovaryc.66_67delAG/p.E23fs (80357713)28 (17)132 (83)
Post-PARPiPeritoneal tumorc.66_67delAG/p.E23fs (80357713)24 (27)66 (73)
4Post first-lineVaginal vault lesionc.3450_3453delCAAG/p.Q1111fs (80357903)50 (12)382 (88)
Post-PARPiPara-aortic LNc.3450_3453delCAAG/p.Q1111fs (80357903)74 (20)298 (80)
5Post-PARPiAscitesc.8975_9100del/p.Pro2992_Gln3034 (80359736)167 (61)104 (38)c.794A>G/p.L265P60 (79)16 (21)
6Post-PARPiAscitesc.1961_1962insA/p.K654fs (80357853)311 (57)232 (43)c.489insTGCTTG/pS166*243 (98)5 (2)

Abbreviation: LN, lymph node.