Table 1.

EZH2 codon 641 mutation variants by adding dNTPs in the designated order (G,A,A,T,C,G,A,T,C) during pyrosequencing

Amino acid changeNucleotide changeSignal strength by nucleotide
Y641TACW-AATACT
W-AATACT___A4 T2 C0 G0 A2 T0 C2
Y641FTAC>TTCW-AATACT
M-AATTCT___A4 T3 C1 G0 A1 T1 C1
Y641STAC>TCCW-AATACT
M-AATCCT___A4 T2 C2 G0 A1 T1 C1
Y641CTAC>TGCW-AATACT
M-AATGCT___A4 T2 C0 G1 A1 T0 C2
Y641NTAC>AACW-AATACT
M-AAAACT___A6 T1 C1 G0 A1 T1 C1
Y641HTAC>CACW-AATACT
M-AACACT___ A4 T1 C1 G0 A2 T0 C2

Abbreviations: W, wild-type; M, mutant; Y, tyrosine.