Table 1.

Summary of 99 consecutive patient cases

Failed initial DNA extraction1
Adequate DNA98
Pretreatment sequencing
 Pretreatment clonal IGH (V–J)91
 Pretreatment clonal IGH (D–J) only1
 Pretreatment biallelic IGH26a
Post-treatment MRD
 HTS+/mpFC+ post-treatment MRD23
 HTS+/mpFC– post-treatment MRD28
 HTS−/mpFC− post-treatment MRD40
Eight additional mpFC-negative cases, triple flow cytometry–sorted for HTS
 Evidence of clonal, pretreatment IGH in sorted mature B-cell fraction1
 No evidence of clonal, pretreatment IGH in sorted mature B-cell fraction in samples with adequate DNA7
  • aAdequate DNA was only available to test for IGH D–J rearrangements (and therefore biallelic samples) in 84 of the 98 cases. Samples with three or more highly expanded IGH sequences were also not considered biallelic.