Table 2.

Summary evolved clonotypes in pretreatment clonal IGH CDR3 sequences

N (%)Mean, median (range)
No VDJ clone7/98 (7.1%)
Pretreatment VDJ clone identified91/98 (92.9%)
No evidence of evolved clonotypes6/91 (6.6%)
Evidence of evolved clonotypes85/91 (93.4%)
Number of evolved clonotypesMean, 195; median, 22 (range, 1–1,953)
Proportion of evolved clonotypes of total cloneMean, 3.4%; median, 0.4%; (range, 0.0010%–59.8%)

NOTE: Clonal IGH CDR3 sequences were identified in nearly all patients as either complete V–DJ or incomplete D–J rearrangements. Among the 91 patients in which a complete V–DJ rearrangement was observed, the number of clones due to ongoing IGH evolution by VH replacement at diagnosis is indicated, as well as the proportion of total clone size (major clone plus all evolved clonotypes) accounted for by these VH-replaced clones. We used the definition of an evolved IGH clonotype initially proposed by Gawad et al. (12).