Table 2.

TLDA 31-gene signature

Gene symbolsGene nameGene locationAUC
Tumor related
 TERCTelomerase RNA component3q260.78
 FOXG1Forkhead box G114q130.95
 PPP1R17Protein phosphatase 1, regulatory subunit 177p150.91
 SLC6A5Solute carrier family 6 (neurotransmitter transporter), member 511p15.10.82
 BCAT1Branched chain amino-acid transaminase 1, cytosolic12p12.10.72
 CBLN3Cerebellin 3 precursor14q120.81
 PID1Phosphotyrosine interaction domain containing 12q36.30.98
 ERG1Early growth response protein 15q23-q310.63
 WIF1WNT inhibitory factor 112q14.30.51
 DKK2Dickkopf WNT signaling pathway inhibitor 24q250.71
 PYGLPhosphorylase, glycogen, liver14q21-q220.51
 TNCTenascin C9q330.82
 PDLIM3PDZ and LIM domain 34q350.89
 HHIPHedgehog interacting protein4q28-q320.93
 SFRP1Secreted frizzled-related protein 18p11.210.90
 GLI1GLI family zinc finger 112q13.2-q13.30.96
 NPR3Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C)5p14-p130.75
 MYCV-myc avian myelocytomatosis viral oncogene homolog8q24.210.52
 IMPG2Interphotoreceptor matrix proteoglycan 23q12.2-q12.30.88
 GABRA5Gamma-aminobutyric acid (GABA) A receptor, alpha 515q120.88
 EOMESEomesodermi3p24.10.88
 MPP3Membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)17q21.310.95
 FSTL5Follistatin-like 54q32.30.84
 PDGFRAPlatelet-derived growth factor receptor, alpha polypeptide4q120.96
 OTX2Orthodenticle homeobox 214q22.30.96
Inflammation related
 CD163CD163 molecule12p13.30.94
 CSF1RColony stimulating factor 1 receptor5q320.78
 MMDMonocyte to macrophage differentiation associated17q220.97
 CD4CD4 molecule12p13.310.74
 CXCR4Chemokine (C–X–C motif) receptor 42q211.00
 ALCAMActivated leukocyte cell adhesion molecule3q13.10.71

NOTE: AUC values for distinguishing WNT and SHH from the Group 3 and 4. Four-way ANOVA P < 0.001 for all genes in signature.