Table 1.

Summary of the NGS results (OncoPanel) on 27 KRAS-mutant NSCLC patients comparing LBK1 genotype with LKB1 IHC staining

Tumor specimenKRAS statusLKB1 status
Biopsy typeTissue of originTumor content (%)Type of mutationAllele fraction (%)CoverageLKB1 DNA changeExonAllele fraction (%)CoverageAA changeLKB1 IHC
SurgicalLung70G12V24138wtIntact
SurgicalLung40G12C9174wtIntact
SurgicalLung70G12C26140wtIntact
SurgicalLung30G12C12277wtLost a
SurgicalLymph node60G12C43223wtLost a
Core bxLung80G12V9135wtIntact
SurgicalAdrenal80Q61H23323wtIntact
SurgicalLung60G12V3281wtIntact
SurgicalLung35G12F40123wtIntact
SurgicalPleura60G12D2999wtIntact
SurgicalLung70G12S28260wtIntact
CytologyLung30G12C15123wtIntact
SurgicalLung70G12C65294wtIntact
SurgicalLung60G12C25212wtIntact
SurgicalLung30G12C31177wtIntact
SurgicalBrain50G12C42171wtIntact
SurgicalPleura50G12V13109c.465_splice + Single copy deletion (19p13.3)1931p.G155_spliceLost
SurgicalLung90G12C45177c.291_splice + Single copy deletion (19p13.3)2687p.K97_spliceLost
SurgicalLymph node80G13C17252c.164_165TG>T + Single copy deletion (19p13.3)133148p.L55fsLost
CytologyPleural fluid60G12C21197c.157_158GA>G121165p.D53fsLost
SurgicalLung50G12D26210c.129_130CA>C146396p.A43fsFocal loss
SurgicalLymph node60G12A19163c.580G>T4965p.D194YLost
SurgicalLymph node70G12C39133c.836_836G>GC64395p.G279fsLost
SurgicalLung90G12V4188c.464_splice5018p.G155_spliceLost
CytologyLung30G12C23296c.598_splice17135p.A200_spliceLost
SurgicalLung70G12V25176Single copy deletion (19p13.3)Focal loss
SurgicalLymph node80G12D9131Single copy deletion (19p13.3)Intact
  • aNo internal control staining.