Table 1.

Cell surface expression of PD-L1 and PD-L1 mRNA abundance in lung cancer cell lines classified according to oncogene status and histology

Cell lineEGFR statusALK statusHistologyPD-L1 mRNAaSurface PD-L1b
HCC827del(E746–A750)Wild-typeAdeno30.93442.74
H1975L858R+T790MWild-typeAdeno21.65355.92
PC9del(E746–A750)Wild-typeAdeno13.41278.39
11_18L858RWild-typeAdeno9.54130.18
H1650del(E746–A750)Wild-typeAdeno3.04111.44
H2228Wild-typeEML4–ALKv3Adeno11.98275.88
H3122Wild-typeEML4–ALKv1Adeno9.86231.03
H322Wild-typeWild-typeAdeno0.7545.23
A549Wild-typeWild-typeAdeno0.7528.17
1-87Wild-typeWild-typeAdeno0.5771.88
LK87Wild-typeWild-typeAdeno0.4626.89
H23Wild-typeWild-typeAdeno0.4214.95
H2122Wild-typeWild-typeAdeno1.8543.93
H1437Wild-typeWild-typeAdeno1.8226.10
H1573Wild-typeWild-typeAdeno0.7635.11
H1944Wild-typeWild-typeAdeno2.2448.13
H157Wild-typeWild-typeAdenosquamous12.61138.37
H596Wild-typeWild-typeAdenosquamous5.3980.87
H460Wild-typeWild-typeLarge cell1.6722.53
H1299Wild-typeWild-typeLarge cell0.9554.44

Abbreviation: Adeno, adenocarcinoma.

  • aPD-L1 mRNA abundance was determined by RT and real-time PCR analysis and was normalized by that of 18S rRNA.

  • bSurface expression of PD-L1 was determined by flow cytometry and is presented as MFI values.