Table 1.

Univariate combined prognostic and predictive effects of TOP1 status in relation to recurrence-free survival and OS

Recurrence-free survivalOS
No.HR (95% CI)PHR (95% CI)P
TOP1 FISH stage II + III534
TOP1 signals per cell
  Continuous0.96 (0.76–1.22)0.760.84 (0.62–1.12)0.22
  Gain vs. normal0.86 (0.60–1.22)0.400.75 (0.49–1.16)0.20
TOP1/CEN20
  Continuous0.90 (0.75–1.09)0.280.74 (0.58–0.95)0.01
  Gain vs. normal1.00 (0.72–1.39)0.990.67 (0.44–1.01)0.06
TOP1 FISH stage III368
TOP1 signals per cell
  Continuous0.96 (0.73–1.27)0.790.81 (0.58–1.13)0.20
  Gain vs. normal0.83 (0.56–1.23)0.350.69 (0.43–1.12)0.14
TOP1/CEN20
  Continuous0.91 (0.74–1.12)0.350.74 (0.56–0.97)0.02
  Gain vs. normal1.04 (0.73–1.48)0.820.70 (0.45–1.09)0.11
TOP1 mRNA expression stage III580
TOP1 mRNA expression
  Continuous0.85 (0.70–1.03)0.100.74 (0.60–0.92)0.007
  High vs. normal0.84 (0.61–1.15)0.280.85 (0.59–1.23)0.38

NOTE: The analyses are made without stratifying for treatment arm. Cutoff values for TOP1 gene gain: ≥ 4 TOP1 signals per cell, TOP1/CEN20 ratio ≥ 1.5. Cutoff value for high TOP1 mRNA expression: ≥ third quartile of the observed TOP1 expression values. The variables are handled linearly in the continuous models.