Table 1.

Characterization of 64Cu-FN3hPD-L1 radiotracer

ParameterResults
FN3hPD-L1 protein production>5 mg/L
Binding affinity (by FACS)1–2 nmol/L
Chemical purity (by HPLC)>95%
DOTA chelates/protein1–2
pH7 ± 0.5
Radiochemical yield>70%
Specific activity (GBq/μmol)5.3 ± 0.5
64Cu-FN3hPD-L1 purity (by TLC and HPLC)>95%
Immunoreactivity fraction85%
  • Abbreviations: DOTA, 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid; FACS, fluorescence activated cell sorter; FN3, fibronectin 3; HPLC, High-performance liquid chromatography; TLC, thin-layer chromatography.