Table 2

Cytoplasmic Pak-1a expression in human colon tissue types (averaged tissue codes) representing neoplastic progression [cytoplasmic histoscores (ScC)]

Pak-1 antibody staining was performed on histologic sections of formalin-fixed, paraffin-embedded surgical specimens. The overall area and intensity of stain was calculated for each tissue and multiplied to give a composite histoscore (i.e. histoscore = area × intensity) for the cytoplasmic stain. Tissues were grouped according to stages of neoplastic progression: normal tissue, intraepithelial neoplasms, invasive neoplasms, and metastases. Repeated measures ANOVA was used to determine differences among tissue type (normal colons, adenomas, invasive CRCs, and lymph node metastases).

Tissue codes averagedbTissue type (n)Pak-1 ScC mean ± SDAdjusted P versus normalcAdjusted P versus adenomascAdjusted P versus invasivec
2, 3Normal (38)0.91 ± 0.41<0.0001<0.0001
5, 6, 7, 8Adenomas (27)1.57 ± 0.45<0.00010.3545
9, 10, 11Invasive CRC (35)1.77 ± 0.44<0.00010.3545
12Lymph node metastases (25)2.26 ± 0.49<0.0001<0.0001<0.0005
  • a Pak-1, p21-activated kinase 1; ScC, composite histoscore; CRC, colorectal cancer.

  • b Normal tissue from patients without inflammatory bowel disease, adenomas, or carcinomas (tissue code 1) was not available.

  • c Adjusted P = P with Tukey adjustment for multiple comparison from ANOVA.