Table 2

Concordance between HER-2 amplification status by two-color FISHa,b and CISH in 192 breast cancers

HER-2 FISH (PathVysion)CISH
No amplification (<6 copies/cell)Amplification (≥6 copies or a gene copy cluster)
HER-2:17 centromere ratio < 2888
HER-2:17 centromere ratio ≥ 2492c
Mean HER-2 count <6 copies/cell8810
Mean HER-2 count ≥6 copies/cell490d
  • a FISH, fluorescence in situ hybridization; CISH, chromogenic in situ hybridization.

  • b Vysis Pathvysion, Abbot Laboratories, Glasgow, Scotland.

  • c Kappa coefficient, 0.875 (0.84–0.91).

  • d Kappa coefficient, 0.854 (0.817–0.891).