Table 1

Oncolytic adenoviruses*

A. Deletion targeting
Oncolytic strainTumor specificModificationsE3 statusCells infectedClinical trialReference
dl1520PanDeleted E1B-55kDDeletionp53 null/mutantPhase I–III (2 , 71 , 75 , 103 104 105 106 107 108 109)
Δ24Deleted E1A CR-2DeletionpRb null/mutantNo (25)
dl922–947Deleted E1A CR-2DeletionpRb null/mutantNo (24)
AxdAdB-3Deleted E1B-55kD, mutated E1ADeletionp53 + pRb null/mutantNo (26)
dl1331Deletion in VAI RNA-coding sequenceIntactRas positiveNo (29)
Adl118BreastE1B-55kD, E1B-19kD deletionsDeletionp53 + pRb null/mutantNo (110)
B. Transcriptional targeting
Oncolytic strainTumor specificPromoterGene controlledE3 statusCells infectedClinical trialReference
Ad5TRE-E1APanTetracycline controlledE1A, E1BDeletionp53 null/mutantNo (42)
Adv-TERTp-E1ATelomerase reverse transcriptase (TERT)E1ADeletionTERT+No (45)
TEhRT-AdTERTE1AIntactTERT+No (111)
HYPR-AdHIF responsiveE1ADeletionHIF+/hypoxicNo (46)
Ad9xHRE1AHIF responsiveE1A, E4IntactHIF+/hypoxicNo (47)
Ar6pAE2fE3FE2F-1E1ADeletionReplicating and pRb null/mutantNo (112)
ONYX-411E2F-1E4, E1AΔCR2DeletionReplicating and pRb null/mutantNo (36)
AdE2F-1RCE2F-1E1ADeletionReplicating and pRb null/mutantNo (43)
01/PEMEp53 responsive elementE2F antagonistDeletionp53 null/mutantNo (44)
AdEHT2Breast1) Estrogen + hypoxia1) E1AIntactEstrogen receptor and TERT +No (30)
2) TERT2) E4
AdEHE2F1) Estrogen + hypoxia1) E1AIntactEstrogen receptor and replicatingNo (30)
2) E2F-12) E4
Ad.ERE2pS2 promoter (estrogen responsive)E1A, E1BIntactEstrogen receptor +No (38)
Ad.DF3-E1DF3/MUC1E1AIntactMUC1+No (31)
AdhOC-E1ProstateBidirectional osteocalcin promoterE1A, E1BIntactOsteocalcin +No (35)
CV7871) Rat probasin1) E1AIntactPSA+Phase I (113)
2) PSA2) E1B
CV7641) Human glandular kallikrein1) E1ADeletionPSA+No (39)
2) PSA2) E1B
CN706PSAE1ADeletionPSA+Phase I (33)
AvE1a04iLiverAFPE1AAFP+No (32)
CV890AFPE1A-IRES-Bicistronic cassetteIntactAFP+No (37)
AdTyrwtMelanomaTyrosinase enhancerE1AIntactTyrosinase+No (40)
AdTyrΔ24Tyrosinase enhancerE1AΔ24 CR-2IntactTyrosinase and pRb null/mutantNo (40)
2) E4
AdTyrΔ2Δ24Tyrosinase enhancerE1A Δ2(NH2 terminus)IntactTyrosinase and pRb null/mutantNo (40)
E1A Δ24
CR-2
KD1-SPBLungSurfactant B1) E1A 01, 07 mutantsDeletionSurfactant B+ and pRb null/mutantNo (41)
2) E4
vCF11ColonTcf responsiveE1AIntactActivated WntNo (114)
AdMKE1NeuroblastomaMidkineE1ADeletionMidkine+No (115)
AdE3-IAI.3BOvarianIAI.3B promoterE1AIntactIAI.3B+No (26)
C. Modified Tropism
Oncolytic strainTumor specificModificationE3 statusCells infectedClinical trialReference
Ad5/3Δ24Ovarian1) Incorporation of serotype 3 knob and retaining Ad5 shaft and tailIntactpRb null/mutantNo (116)
2) 24 bp deletion in CR2 of E1A
  • Abbreviations: HIF, hypoxia-inducible factor.

  • * Revised and reprinted with permission from Ref. 1 .

  • Refer to cited reference for extent of E3 deletions for each virus.