Table 3

Individual cases determined to be micrometastasis positive in lymph node

CaseBlue-dyed nodeNondyed lymph nodeInvasion depthHistologic findings
CK20 (RT-PCR)CEA (RT-PCR)Micro-metastasisCK (IHC)CK20 (RT-PCR)CEA (RT-PCR)Micro-metastasisCK (IHC)Lymphatic invasionVascular invasionHistology
11/2*1/2+1/20/30/30/3smtub
20/21/2+0/20/30/30/3mtub
31/41/4+0/40/50/50/5ss++por
41/61/6+0/60/60/60/6smsig
51/30/3+1/30/30/30/3smtub
60/21/2+0/20/40/40/4mtub
73/53/5+1/50/50/50/5smpor
82/20/2+0/22/40/4+0/4ss+tub
92/40/4+2/41/40/4+1/4smtub
101/62/6+1/60/33/3+1/3mpor
  • NOTE. +, positive; −, negative.

  • Abbreviations: m, mucosa; sm, submucosa; ss, subserosa; tub, tubular adenocarcinoma; por, poorly differentiated adenocarcinoma; sig, signet ring cell carcinoma.

  • * Number of positive lymph node/total lymph node number.

  • Micrometastasis was determined to be positive when a lymph node expressed either CK20 or CEA.

  • Immunohistochemistry by cytokeratin staining.