Table 2

The highly or specifically expressed proteins in myeloid, granulocytic, monocytic, and lymphoid lineage of acute leukemia identified by MALDI-TOF-MS

SpotProtein nameNCBInr ID no.MrpIPeptides (MALDI/MS)Sequence coverage (%)Score
TheorObservTheorObservMatchTotal
M-1Chain A, structure of up1-telomeric DNA complexgi 554253020,83122,6456.796.8912394982
M-2Unnamed protein productgi 1655301620,61121,4359.605.016143264
M-3Coactosin-like 1gi 2162460715,93516,8875.545.397185282
M-4Cytochrome c oxidase subunit VIbgi 450298510,18612,2016.306.545136577
M-5F-actin capping protein β subunit; Cap Zgi 482665930,60934,0765.695.52143253137
M-6Chain A, crystal structure of the Mrp14 complexed with CHAPSgi 2015022913,10214,8215.715.46155382106
M-7S100 calcium-binding protein A8 (Mrp8)gi 2161454410,82812,9506.517.2761260103
M-8Nit protein 2gi 991046030,58935,1596.827.12161973207
M-9Similar to ATP synthase, H+ transporting, mitochondrial F1 complex,gi 1311190140,26033,4208.947.119182874
M-10*Glyoxalase Igi 213530120,76427,9885.124.939255377
M-11*Phosphoglycerate kinase 1gi 450576344,58638,6988.306.8910154097
M-12UP1, the two RNA-recognition motif domain of HnrnpA1gi 255465320,78420,3467.267.4682363136
M-13Proteasome β subunitgi 63134525,89328,9125.705.7810174684
M-14Adenylate kinase, mitochondrial; ATP-AMP transphosphorylasegi 752434625,59833,1937.718.216112665
M-15Aldolase Agi 22967439,26431,7268.397.43122846137
M-16Chain A, myloperoxidasegi 49439412,31115,3635.776.47143096185
M-17Triosephosphate isomerasegi 99989226,22126,8996.517.10113550149
M-18Ubiquinol-cytochrome c reductasegi 517474329,63326,3248.557.1210513379
M-19Cathepsin Dgi 49429626,22930,9585.315.8010262525
G-1Similar to ribosomal protein S12; 40S ribosomal protein S12gi 2748548814,58914,3906.436.408235090
G-2CDC42 GAP-related proteingi 232617125,20932,6619.387.607203570
G-3Fatty acid binding protein 5 (psoriasis-associated); E-FABPgi 455758115,15516,4806.606.4110285680
G-4Actin γgi 7162541,63540,1935.315.49225366126
G-5α enolasegi 266103936,28637,6356.536.68194263176
G-6γ-actingi 17804525,86230,6165.655.4111493971
G-7β5-tubulingi 1808871949,64040,8224.755.78205568126
G-8Chain A, myloperoxidasegi 49439412,31115,3635.776.47143096185
G-9*Similar to VENT-like homeobox 2; haemopoietic progenitor homeoboxgi 3015362636,67736,8898.987.58581764
G-103-Hydroxyacyl-CoA dehydrogenase, isoform 2gi 207832942,09736,9679.347.656102675
G-11*Peroxiredoxin 2; thiol-specific antioxidant protein (TSA)gi 250716921,87825,6765.665.237195693
G-12P47gi 553182740,54737,7105.035.32134740125
M5-1Adenine phosphoribosyltransferase; AMP pyrophosphorylasegi 450217119,59518,3725.785.245941
M5-2Annexin A2; annexin II (lipocortin II)gi 1630697838,59437,7317.577.028112865
M5-3HSPC124gi 684147036,51936,6895.606.067132993
M5-4Sulfotransferase family, cytosolic, 1Agi 1083503534,17436,6895.685.6510504163
M5-5Unnamed protein productgi 2175857832,53032,7906.716.7017406371
M5-6Inorganic pyrophosphatase 2 isoform 2gi 2917169431,55636,6897.046.2161129150
M5-7Hypothetical proteingi 1205283436,54136,6895.966.548143982
M5-8Unknown (protein for MGC:27221)gi 1692426535,73534,9868.476.70153956103
M5-9Transgelin 2gi 1280356722,39222,8758.418.45153253127
L-1Immunoglobulin heavy chaingi 80683213,70414,2148.577.05496574
L-2*Stathmin; leukemia-associated phosphoprotein p18 (Op18)gi 503185117,29219,6995.764.989224673
L-3*Heat shock 27kDa protein 1gi 450451722,76825,5345.986.01175177168
L-4Aldehyde reductasegi 163330036,41935,2566.346.64173855160
L-5Endoplasmic reticulum protein 29 precursorgi 580301328,97523,8646.776.76133255126
L-6Cofilin 1 (non-muscle)gi 503163518,49120,3218.226.4111455675
  • NOTE. The calculation of experimental isoelectric point (pI) and Mr was based on migration of the protein spot on two-dimensional gels. The highly or specifically expressed proteins in myeloid, granulocytic, monocytic, and lymphoid lineage of acute leukemia, as compared with other subtypes, were designated with the letter M, G, M5, and L, respectively. A score of more than 63 is significant (P < 0.05).

  • Abbreviations: ID, identification; Theor, theoretical; Observ, observed.

  • * The proteins that had been reported to be involved in leukemogenesis.

  • The proteins that had been reported to be associated with acute leukemia classification.