Table 1

Design and location of the oligonucleotides used for quantitative methylation-sensitive real-time PCR

GeneAlternate nameGenBank acession no.Amplicon location (relative to transcription start sites)Location of amplicon
ARF p14AF082338−203/−135Promoter
FADD Fas (TNFRSF6)-associated via death domainNM_003824−138/−78Promoter
TNFRSF21 TNF receptor superfamily, member 2NM_014452−426/−327Promoter
LITAF LPS-induced TNF-α factorNM_004862−238/−143Promoter
BAX BCL2-associated X protein, isoform βNM_138762+91/+169Exon 1
TMS1 Apoptosis-associated speck-like protein (ASC)NM_013258+25/+105Exon 1
DAPK Death-associated protein kinase 1X76104+137/+204Exon 1
TERT TelomeraseAF325900−223/−108Promoter
BCL2 bcl-2 (B-cell lymphoma type 2)NM_000633+210/+293Exon 1
EDNRB EDNRB, endothelin receptor B, CpG island 2NM_003991+12/+104Exon 1
RASSF1A Ras association (RalGDS/AF-6) domain family 1NM_007182+57/+121Exon 1
TNFRSF25 Death receptor 3AB051850+27/+95Exon 1
ACTB β-Actin
COL2A1 Collagen type 2L10347
  • Abbreviations: TNF, tumor necrosis factor; LPS, lipopolysaccharide.