Table 1

Flow cytometry analysis of control and myrAkt1 MCF-7 cells exposed to different treatments

ControlmyrAkt1
ControlRapa (20 nmol/L)Tam (10−7)Rapa + TamControlRapa (20 nmol/L)Tam (10−7)Rapa + Tam
G127 ± 2 *23 ± 325 ± 225 ± 145 ± 446 ± 344 ± 141 ± 2
S54 ± 357 ± 257 ± 152 ± 236 ± 137 ± 340 ± 339 ± 2
G218 ± 320 ± 118 ± 223 ± 319 ± 317 ± 116 ± 220 ± 1
Sub-G118 ± 223 ± 335 ± 339 ± 316 ± 215 ± 114 ± 326 ± 1
  • NOTE. Control and myrAkt1 MCF-7 cells were exposed for 24 hours to either vehicle (control), 20 nmol/L rapamycin (Rapa), 10-7 mol/L tamoxifen (Tam) or the combination of the two, then assessed for cell cycle status, as well as apoptotic (sub-G1) status.

  • * Each value represents the average ± SD of three independent observations.