Table 2

PCR protocols for HER-2

PCR stepHER-2 1st PCRNo of cyclesHER-2 2nd PCRNo of cycles
Denaturation6 min/94°C13 min/94°C1
50 s/94°C130 s/94°C5
30 s/72°C
30 s/94°C5
30 s/70°C
30 s/72°C
30 s/94°C5
30 s/68°C
30 s/72°C
Amplification3530 s/94°C5
2:30 min/58°C30 s/66°C
30 s/72°C
30 s/94°C5
30 s/64°C
30 s/72°C
30 s/94°C15
30 s/62°C
30 s/72°C
Final extension10 min/72°C17 min/72°C1