Table 1

c-Kit amplification primers

NamePositionTemperature of annealing (°C)Fragment
Kit a (forward): 5′-CTGCACTTGGGCGAGAGC-3′−81 from the ATG591
Kit a (reverse): 5′-AGAACAATGCAGACAGAGCCG-3′561
Kit b (forward): 5′-CAGACCCAGAAGTGACC-3′416542
Kit b (reverse): 5′-ACTGCTGGTGTTCAGGTTTGG-3′1042
Kit c (forward): 5′-GCAAATGTCACAACAACC-3′895523
Kit c (reverse): 5′-ATTCAACCGTGCCATTGTGC-3′1471
Kit d (forward): 5′-AACTGAGCAGAGATGC-3′1335504
Kit d (reverse): 5′-TGATTACCAAGGTAACTCAGG-3′1928
Kit e (forward): 5′-TGAGGCAACTGCTTATGG-3′1812555
Kit e (reverse): 5′-GCTCCCAAAGAAAAATCCCATAGG-3′2584
Kit f (forward): 5′-GAGTTGGCCCTAGAGTTAGA-3′2281636
Kit f (reverse): 5′-CCTGGAGTTGGATGCAAGTT-3′3029
Kit g (forward): 5′-AACGAGATTAAAAGGCACC-3′1137537
Kit g (reverse): 5′-AGCTACGAT TACGAAACC-3′1609
Kit h (forward): 5′-ACCCTGTTCACTCCTTTG-3′1558538
Kit h (reverse): 5′-TTACAGCGACAGTCATGG-3′1867
Kit i (forward): 5′-TGAGGCAACTGCTTATGG-3′1821539
Kit i (reverse): 5′-ACAACATAAGAAACTCCAG-3′2195
  • NOTE. The primers used for the amplifications of whole c-Kit cDNA.