Table 2

Characterization of 120 melanomas for NY-ESO-1 and LAGE-1 by immunohistochemistry, RT-PCR, and qRT-PCR

A. E978 antibody staining categorized on the basis of NY-ESO-1 and LAGE-1 expression
Tumors typed by RT-PCRTumors typed by qRT-PCR*
mRNANumberPositive by mAb E978 (%)NumberPositive by mAb E978 (%)
NY-ESO-1+/LAGE-1+3733 (89)3028 (93)
NY-ESO-1+/LAGE-1−1110 (91)2015 (75)
NY-ESO-1-/LAGE-1 +152 (13.3)100 (0)
NY-ESO-1 -/LAGE-1−579 (15.5)6011 (18)
Total12054 (45)12054 (45)
B. Comparison of RT-PCR and immunohistochemistry based on levels of NY-ESO-1 antigen expression
NY-ESO-1 immunohistochemistry with mAb E978: % cells positive
RT-PCRA >50% (n = 23)B 11–50% (n = 15)C 1–10% (n = 16)D Negative (n = 66)Total
+++1752125
++654217
+01326
Negative0476172
Total23151666120
  • NOTE. +++, strong band seen on gel; ++, distinctive band seen on gel; +, weak band seen on gel; −, no product seen on gel.

  • * Positive by qRT-PCR defined as >25 copies mRNA.