Table 1

Primer sequences

PrimerSequence
Ras A5′-ACTGAATATAAACTTGTGGTCCATGGAGCT-3′a
Ras B5′-TTATCTGTATCAAAGAATGGTCCTGCACCA-3′
Ras C5′-GGATGGTCCTCCACCAGTAATATGGATATTA-3′a
RasSeq5′-ACCTCTATTGTTGGATCATA-3′
IS-A(5′)5′-GGT GGA GTA TTT GAT AGT GTA TTA ACC TTA TGT G-3′
IS-A(3′)5′-AAT TAA GCT TAT CGT CAA GGC ACT CTT GCC-3′b
IS-B(5′)5′-AAT TAA GCT TCA TTT TGT GGA CGA ATA TG-3′b
IS-B(3′)5′-GGT CAG AGA AAC CTT TAT CTG TAT CAA A-3′
IS-C(5′)5′-GTG TAT TAA CCT TAT GTG TGA C-3′
IS-C(3′)5′-CAG AGA AAC CTT TAT CTG TAT C-3′
  • a Mismatch bases are underlined.

  • b Base sequence for restriction enzyme HindIII is underlined.